{"created":"2023-07-27T06:26:34.046133+00:00","id":10336,"links":{},"metadata":{"_buckets":{"deposit":"8da6dd5c-1743-4d89-bede-c98aec2c0ba2"},"_deposit":{"created_by":3,"id":"10336","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"10336"},"status":"draft"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00010336","sets":["934:935:937"]},"author_link":["15562","15563","15564","15565","15084"],"control_number":"10336","item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1991-01-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"353","bibliographicPageStart":"348","bibliographicVolumeNumber":"109","bibliographic_titles":[{"bibliographic_title":"Journal of Biochemistry"}]}]},"item_4_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"板垣, 英治"}],"nameIdentifiers":[{}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Salicylate hydroxylase [EC 1.14.13.1] from Pseudomonas putida catalyzed the formation of catechol from substrate analogues such as o-nitro-, o-amino-, o-iodo-, o-bromo-, and o-chloro-phenol by removing the ortho-substituted groups.They are converted into nitrite, ammonia, and halide ions, respectively. Kinetic parameters of these reactions were determined by spectrophotometric and polarographic methods. Hydroxylation of o-nitro- or o-iodophenol proceeds with the unusual stoichiometry of 2 : 1 : 1 for consumed NADH, O2-uptake, and catechol formed. Other ortho-substituted phenols examined also gave the same results. Like salicylate, these substrates perturb the absorption spectrum of salicylate hydroxylase in the visible region, indicating the formation of enzyme·substrate complexes. Titration experiments with ortho-substituted phenols gave the dissociation constants of the complexes. The complexes were quantitatively reduced with NADH or dithionite without detectable formation of the intermediates. The fact that one atom of 18O2 was incorporated into the produced catechol in hydroxylation of o-nitrophenol indicates that the reaction is of monooxygenase nature. It is concluded that salicylate hydroxylase cleaves the C-N and C-X bonds of ortho-substituted phenols.","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学自然科学研究科　　","subitem_description_type":"Other"},{"subitem_description":"金沢大学理工研究域自然システム学系","subitem_description_type":"Other"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本生化学会 = Japanese Biochemical Society"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1093/oxfordjournals.jbchem.a123384","subitem_relation_type_select":"DOI"}}]},"item_4_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://jb.oxfordjournals.org/cgi/content/abstract/109/2/348","subitem_relation_type_select":"URI"}}]},"item_4_rights_23":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright © 1991 Japanese Biochemical Society"}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA00694073","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0021-924X","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Suzuki, Kenzi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Gomi, Tomoharu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kaidoh, Toshio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Itagaki, Eiji"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-03"}],"displaytype":"detail","filename":"SC-PR-ITAGAKI-E-348.pdf","filesize":[{"value":"830.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"SC-PR-ITAGAKI-E-348.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/10336/files/SC-PR-ITAGAKI-E-348.pdf"},"version_id":"5c55fe4b-c9d6-4a67-8d03-a3eaee1b54e2"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Hydroxylation of o-halogenophenol and o-nitrophenol by salicylate hydroxylase","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Hydroxylation of o-halogenophenol and o-nitrophenol by salicylate hydroxylase"}]},"item_type_id":"4","owner":"3","path":["937"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-10-03"},"publish_date":"2017-10-03","publish_status":"0","recid":"10336","relation_version_is_last":true,"title":["Hydroxylation of o-halogenophenol and o-nitrophenol by salicylate hydroxylase"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-12-19T02:36:23.412869+00:00"}