{"created":"2023-07-27T06:26:34.173582+00:00","id":10339,"links":{},"metadata":{"_buckets":{"deposit":"e7e2e3d8-20bf-4b81-a2a5-448d1dc29432"},"_deposit":{"created_by":3,"id":"10339","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"10339"},"status":"draft"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00010339","sets":["934:935:937"]},"author_link":["15573","15574","15575","15084"],"control_number":"10339","item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1991-01-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"797","bibliographicPageStart":"791","bibliographicVolumeNumber":"109","bibliographic_titles":[{"bibliographic_title":"Journal of Biochemistry"}]}]},"item_4_creator_36":{"attribute_name":"著者別名","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"板垣, 英治"}],"nameIdentifiers":[{"nameIdentifier":"15084","nameIdentifierScheme":"WEKO"}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Salicylate hydroxylase [EC 1.14.13.1] from Pseudomonas putida catalyzes the hydroxylation of salicylate, and also o-aminophenol, o-nitrophenol, and o-halogenophenols, to catechol. The reactions with these o-substituted phenols comprise oxygenative deamination, denitration, and dehalogenation, respectively. The reaction try, as to NADH oxidized, oxygen consumed, and catechol formed, is 2 : 1 : 1, respectively. The mechanisms for the deiodination and oxygenation of o-iodophenol were investigated in detail by the use of I+-trapping reagents such as DL-methionine, 2-chlorodimedone, and L-tyrosine. The addition of the traps did not change the molar ratio of catechol formed to NADH oxidized, nor iodinated traps produced were in the incubation mixture. The results suggest that I+ was not produced on the deiodination in the hydroxylation of o-iodophenol. On the other hand, L-ascorbate, L-epinephrine, and phenylhydrazine increased the molar ratio. o-Phenylenediamine decreased it, being converted to phenazine. This suggests that o-benzoquinone is formed in the oxidation of o-iodophenol as a nascent product. The quinone was detected spectrophotometrically by means of the stopped-flow method. Kinetic analysis of the reactions revealed that o-benzoquinone is reduced nonenzymatically to catechol by a second molecule of NADH. A mechanism of elimination for the ortho-substituted groups of substrate phenols by the enzyme is proposed and discussed.","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学自然科学研究科　　","subitem_description_type":"Other"},{"subitem_description":"金沢大学理工研究域自然システム学系","subitem_description_type":"Other"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本生化学会 = Japanese Biochemical Society"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1093/oxfordjournals.jbchem.a123458","subitem_relation_type_select":"DOI"}}]},"item_4_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://jb.oxfordjournals.org/cgi/content/abstract/109/5/791","subitem_relation_type_select":"URI"}}]},"item_4_rights_23":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright © 1991 Japanese Biochemical Society"}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA00694073","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0021-924X","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Suzuki, Kenzi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Gomi, Tomoharu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Itagaki, Eiji"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-03"}],"displaytype":"detail","filename":"SC-PR-ITAGAKI-E-791.pdf","filesize":[{"value":"1.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"SC-PR-ITAGAKI-E-791.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/10339/files/SC-PR-ITAGAKI-E-791.pdf"},"version_id":"5a5b8f04-954f-4953-a260-f77045478482"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Intermediate and mechanism of hydroxylation of o-iodophenol by salicylate hydroxylase","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Intermediate and mechanism of hydroxylation of o-iodophenol by salicylate hydroxylase"}]},"item_type_id":"4","owner":"3","path":["937"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-10-03"},"publish_date":"2017-10-03","publish_status":"0","recid":"10339","relation_version_is_last":true,"title":["Intermediate and mechanism of hydroxylation of o-iodophenol by salicylate hydroxylase"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-12-19T02:36:31.239199+00:00"}