{"created":"2023-07-27T06:26:34.894108+00:00","id":10357,"links":{},"metadata":{"_buckets":{"deposit":"d2f920c3-f201-4cdb-8200-43fecaf89c73"},"_deposit":{"created_by":3,"id":"10357","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"10357"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00010357","sets":["934:935:937"]},"author_link":["15622","13404","15624","15618","15619","15621","15620","15623"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-01-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"10","bibliographicPageStart":"e13240","bibliographicVolumeNumber":"5","bibliographic_titles":[{"bibliographic_title":"PLoS ONE"}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Formation of fibrillar structures of proteins that deposit into aggregates has been suggested to play a key role in various neurodegenerative diseases. However mechanisms and dynamics of fibrillization remains to be elucidated. We have previously established that lithostathine, a protein overexpressed in the pre-clinical stages of Alzheimer's disease and present in the pathognomonic lesions associated with this disease, form fibrillar aggregates after its N-terminal truncation. In this paper we visualized, using high-speed atomic force microscopy (HS-AFM), growth and assembly of lithostathine protofibrils under physiological conditions with a time resolution of one image/s. Real-time imaging highlighted a very high velocity of elongation. Formation of fibrils via protofibril lateral association and stacking was also monitored revealing a zipper-like mechanism of association. We also demonstrate that, like other amyloid ß peptides, two lithostathine protofibrils can associate to form helical fibrils. Another striking finding is the propensity of the end of a growing protofibril or fibril to associate with the edge of a second fibril, forming false branching point. Taken together this study provides new clues about fibrillization mechanism of amyloid proteins. © 2010 Milhiet et al.","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学理工研究域数物科学系","subitem_description_type":"Other"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Public Library of Science"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1371/journal.pone.0013240","subitem_relation_type_select":"DOI"}}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1932-6203","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Milhiet, Pierre-Emmanuel"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamamoto, Daisuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Berthoumieu, Olivia"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Dosset, Patrice"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Grimellec, Christian Le"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Verdier, Jean-Michel"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Marchal, Stéphane"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ando, Toshio"}],"nameIdentifiers":[{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-03"}],"displaytype":"detail","filename":"SC-PR-ANDO-T-13240.pdf","filesize":[{"value":"408.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"SC-PR-ANDO-T-13240.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/10357/files/SC-PR-ANDO-T-13240.pdf"},"version_id":"d3515e7a-8d08-40de-b4dd-0e98773cdde8"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Deciphering the structure, growth and assembly of amyloid-like fibrils using high-speed atomic force microscopy","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Deciphering the structure, growth and assembly of amyloid-like fibrils using high-speed atomic force microscopy"}]},"item_type_id":"4","owner":"3","path":["937"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-03"},"publish_date":"2017-10-03","publish_status":"0","recid":"10357","relation_version_is_last":true,"title":["Deciphering the structure, growth and assembly of amyloid-like fibrils using high-speed atomic force microscopy"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-07-28T01:41:50.963049+00:00"}