{"created":"2023-07-27T06:26:37.487320+00:00","id":10420,"links":{},"metadata":{"_buckets":{"deposit":"c5a0fcf4-c7ab-4972-8afe-6efd143385ae"},"_deposit":{"created_by":3,"id":"10420","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"10420"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00010420","sets":["934:935:937"]},"author_link":["15783","15083","15782","15785","15784","15780","15781"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-02-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"167","bibliographicPageStart":"159","bibliographicVolumeNumber":"115","bibliographic_titles":[{"bibliographic_title":"Journal of Bioscience and Bioengineering"}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The flac1 gene consisted of 1488 bases encodes a novel laccase (Flac1) from Flammulina velutipes. The deduced amino acid sequence of Flac1 with 496 amino acids shows 58-64% homologies with other fungal laccases. The recombinant Flac1 (rFlac1) was heterologously expressed in Pichia pastoris, with sugars of approximately 4 kDa attached on the protein molecule, which has the calculated molecular mass of 53,532 Da. rFlac1 was shown to be a multi-copper oxidase from spectroscopies. The optimum pHs of rFlac1 for oxidations of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), p-phenylenediamine, and o-aminophenol, were 5.0, 5.0, and 6.0-6.5, respectively, showing higher pH values than those from many other fungal laccases. The slightly acidic or neutral optimum pH that is not strongly dependent on substrates is a unique property of rFlac1. Effective O2 reduction was realized by the direct electron transfer of rFlac1 at a highly oriented pyrolytic graphite electrode modified with fine carbon particles (Ketjen Black) in O2-saturated solution. The pHs showing the maximum ΔE°' [= E°'(enzyme) - E°'(substrate)] coincided well with the optimum pHs shown by rFlac1 under steady-state conditions. The present electrochemical results of rFlac1 indicate that ΔE°' is one of the primary factors to determine the activity of multi-copper oxidases. © 2012 The Society for Biotechnology, Japan","subitem_description_type":"Abstract"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Society for Biotechnology, Japan (日本生物工学会) / Elsevier"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.jbiosc.2012.09.011","subitem_relation_type_select":"DOI"}}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA11307678","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1389-1723","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Saito-Otsuka, Kaori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kurose, Shinji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tsujino, Yoshio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Osakai, Toshiyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kataoka, Kunishige"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sakurai, Takeshi"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Tamiya, Eiichi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-03"}],"displaytype":"detail","filename":"SC-PR-SAKURAI-T-159.pdf","filesize":[{"value":"1.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"SC-PR-SAKURAI-T-159.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/10420/files/SC-PR-SAKURAI-T-159.pdf"},"version_id":"671d0c09-a4fe-4ae3-a80f-8fab598eddde"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Electrochemical characterization of a unique, \"neutral\" laccase from Flammulina velutipes","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Electrochemical characterization of a unique, \"neutral\" laccase from Flammulina velutipes"}]},"item_type_id":"4","owner":"3","path":["937"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-03"},"publish_date":"2017-10-03","publish_status":"0","recid":"10420","relation_version_is_last":true,"title":["Electrochemical characterization of a unique, \"neutral\" laccase from Flammulina velutipes"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-07-28T01:41:08.838013+00:00"}