@article{oai:kanazawa-u.repo.nii.ac.jp:00010429,
 author = {五十嵐, 圭日子 and 内橋, 貴之 and 鮫島, 正浩 and Igarashi, Kiyohiko and Uchihashi, Takayuki and Koivula, Anu and Wada, Masahisa and Kimura, Satoshi and Okamoto, Tetsuaki and Penttilä, Merja and Ando, Toshio and Samejima, Masahiro},
 issue = {6047},
 journal = {Science},
 month = {Sep},
 note = {Abstract A deeper mechanistic understanding of the saccharification of cellulosic biomass could enhance the efficiency of biofuels development. We report here the real-time visualization of crystalline cellulose degradation by individual cellulase enzymes using an advanced version of high-speed atomic force microscopy. Trichoderma reesei 2 cellobiohydrolase I (TrCel7A) molecules were observed to slide unidirectionally along the crystalline cellulose surface, but at one point exhibited collective halting analogous to a traffic jam. Changing the crystalline polymorphic form of cellulose by an ammonia treatment increased the apparent number of accessible lanes on the crystalline surface and consequently the number of moving cellulase molecules. Treatment of this bulky crystalline cellulose simultaneously or separately with T. reesei cellobiohydrolase II (TrCel6A) resulted in a significant increase in the proportion of mobile enzyme molecules on the surface. Cellulose was completely degraded by the synergistic action between the two enzymes., 金沢大学ナノ生命科学研究所 / 金沢大学理工研究域数物科学系},
 pages = {1279--1282},
 title = {Traffic Jams Reduce Hydrolytic Efficiency of Cellulase on Cellulose Surface},
 volume = {333},
 year = {2011}
}