{"created":"2023-07-27T06:26:37.870740+00:00","id":10429,"links":{},"metadata":{"_buckets":{"deposit":"496eb783-5275-48ac-be5a-42aeb4700f27"},"_deposit":{"created_by":3,"id":"10429","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"10429"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00010429","sets":["4161:3020:3021"]},"author_link":["15810","15814","15812","1518","15813","15808","2912","13404","1042","15811","15809","98"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-09-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6047","bibliographicPageEnd":"1282","bibliographicPageStart":"1279","bibliographicVolumeNumber":"333","bibliographic_titles":[{"bibliographic_title":"Science"}]}]},"item_4_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"五十嵐, 圭日子"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"内橋, 貴之"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"鮫島, 正浩"}],"nameIdentifiers":[{},{},{},{}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Abstract A deeper mechanistic understanding of the saccharification of cellulosic biomass could enhance the efficiency of biofuels development. We report here the real-time visualization of crystalline cellulose degradation by individual cellulase enzymes using an advanced version of high-speed atomic force microscopy. Trichoderma reesei 2 cellobiohydrolase I (TrCel7A) molecules were observed to slide unidirectionally along the crystalline cellulose surface, but at one point exhibited collective halting analogous to a traffic jam. Changing the crystalline polymorphic form of cellulose by an ammonia treatment increased the apparent number of accessible lanes on the crystalline surface and consequently the number of moving cellulase molecules. Treatment of this bulky crystalline cellulose simultaneously or separately with T. reesei cellobiohydrolase II (TrCel6A) resulted in a significant increase in the proportion of mobile enzyme molecules on the surface. Cellulose was completely degraded by the synergistic action between the two enzymes.","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学ナノ生命科学研究所 / 金沢大学理工研究域数物科学系","subitem_description_type":"Other"}]},"item_4_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00010416","subitem_identifier_reg_type":"JaLC"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"American Association for the Advancement of Science"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1126/science.1208386","subitem_relation_type_select":"DOI"}}]},"item_4_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.sciencemag.org/content/333/6047/1279.full","subitem_relation_type_select":"URI"}}]},"item_4_rights_23":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2011 American Association for the Advancement of Science."}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA00835277","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0036-8075","subitem_source_identifier_type":"ISSN"}]},"item_4_text_2":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_text_value":"セルロース表面におけるセルラーゼの交通渋滞が加水分解効率を低下させる"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Igarashi, Kiyohiko"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Uchihashi, Takayuki"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Koivula, Anu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Wada, Masahisa"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kimura, Satoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Okamoto, Tetsuaki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Penttilä, Merja"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ando, Toshio"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Samejima, Masahiro"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-03"}],"displaytype":"detail","filename":"SC-PR-UCHIHASHI-T-1279.pdf","filesize":[{"value":"4.4 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"SC-PR-UCHIHASHI-T-1279.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/10429/files/SC-PR-UCHIHASHI-T-1279.pdf"},"version_id":"5572b610-13d4-4362-8f53-7a5c27e933f9"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Traffic Jams Reduce Hydrolytic Efficiency of Cellulase on Cellulose Surface","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Traffic Jams Reduce Hydrolytic Efficiency of Cellulase on Cellulose Surface"}]},"item_type_id":"4","owner":"3","path":["3021"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-03"},"publish_date":"2017-10-03","publish_status":"0","recid":"10429","relation_version_is_last":true,"title":["Traffic Jams Reduce Hydrolytic Efficiency of Cellulase on Cellulose Surface"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-07-27T10:16:09.162488+00:00"}