| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2017-10-03 |
| タイトル |
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|
タイトル |
A marine phytoplankton (Prymnesium parvum) up-regulates ABC transporters and several other proteins to acclimatize with Fe-limitation |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| ID登録 |
|
|
ID登録 |
10.24517/00010566 |
|
ID登録タイプ |
JaLC |
| 著者 |
Mamunur, Rahman M.
Azizur, Rahman M.
Maki, Teruya
Nishiuchi, Takumi
Asano, Tomoya
Hasegawa, Hiroshi
|
| 著者別表示 |
牧, 輝弥
西内, 巧
浅野, 智哉
長谷川, 浩
|
| 書誌情報 |
Chemosphere
巻 95,
p. 213-219,
発行日 2014-01-01
|
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0045-6535 |
| NCID |
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収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00603442 |
| DOI |
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関連タイプ |
isVersionOf |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
10.1016/j.chemosphere.2013.09.001 |
| 出版者 |
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出版者 |
Elsevier |
| 抄録 |
|
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内容記述タイプ |
Abstract |
|
内容記述 |
Iron (Fe) is one of the vital limiting factors for phytoplankton in vast regions of the contemporary oceans, notably the high nutrient low chlorophyll regions. Therefore, it is apparent to be acquainted with the Fe uptake strategy of marine phytoplankton under Fe-limited condition. In the present study, marine phytoplankton Prymnesium parvum was grown under Fe-deplete (0.0025 μM) and Fe-rich (0.05 μM) conditions, and proteomic responses of the organism to Fe conditions were compared. In sodium dodecyl sulfate (SDS) gel electrophoresis, 7 proteins (16, 18, 32, 34, 75, 82, and 116. kDa) were highly expressed under Fe-deplete condition, while one protein (23. kDa) was highly expressed under Fe-rich condition. These proteins were subjected to 2-dimensional gel electrophoresis (2-D DIGE) to differentiate individual proteins, and were identified by matrix-assisted laser desorption-ionization-time of flight-mass spectrometer (MALDI-TOF-MS) analysis. The results showed that under Fe-deplete condition P. parvum increases the biosynthesis of ATP binding cassette (ABC) transporters, flagellar associated protein (FAP), and Phosphoribosylaminoimidazole-succinocarboxamide synthase. These proteins are assumed to be involved in a number of cellular biochemical processes that facilitate Fe acquisition in phytoplankton. Under Fe-deplete condition, P. parvum increases the synthesis of ribulose biphosphate carboxylase (RuBisCo), malate dehydrogenase, and two Fe-independent oxidative stress response proteins, manganese superoxide dismutase (MnSOD) and Serine threonine kinase (STK). Thus, marine phytoplankton may change their Fe acquisition strategy by altering the biosynthesis of several proteins in order to cope with Fe-limitation. © 2013 Elsevier Ltd. |
| 著者版フラグ |
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出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
SC-PR-HASEGAWA-H-213.pdf (537.9 kB)
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