{"created":"2023-07-27T06:26:46.640518+00:00","id":10636,"links":{},"metadata":{"_buckets":{"deposit":"46c25ee2-9ce4-4f0d-92a9-fe11a30e32fa"},"_deposit":{"created_by":3,"id":"10636","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"10636"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00010636","sets":["934:935:937"]},"author_link":["16209","16211","15508","16210"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-01-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"252","bibliographicPageStart":"243","bibliographicVolumeNumber":"54","bibliographic_titles":[{"bibliographic_title":"The Journal of General and Applied Microbiology"}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The cyanobacterium Nostoc commune is adapted to the terrestrial environment and forms a visible colony in which the cells are embedded in extracellular polysaccharides (EPSs), which play a crucial role in the extreme desiccation tolerance of this organism. When natural colonies were immersed in water, degradation of the colonies occurred within 2 days and N. commune cells were released into the water. The activities that hydrolyze glycoside bonds in various N. commune fractions were examined using artificial nitrophenyl-linked sugars as substrates. A β-D-glucosidase purified from the water-soluble fraction was resistant to 20 min of boiling. The β-D-glucosidase, with a molecular mass of 20 kDa, was identified as a cyanobacterial fasciclin protein based on its N-terminal amino-acid sequence. The 36-kDa major protein in the water-soluble fraction was purified, and the N-terminal amino-acid sequence of the protein was found to be identical to that of the water-stress protein (WspA) of N. commune. This WspA protein also showed heat-resistant β-D-galactosidase activity. The fasciclin protein and WspA in the extracellular matrix may play a role in the hydrolysis of the EPSs surrounding the cells, possibly as an aid in the dispersal of cells, thus expanding the colonies of this cyanobacterium.","subitem_description_type":"Abstract"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"応用微生物学・分子細胞生物学研究奨励会 = The Applied Microbiology, Molecular and Cellular Biosciences Research Foundation"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.2323/jgam.54.243","subitem_relation_type_select":"DOI"}}]},"item_4_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://www.jstage.jst.go.jp/browse/jgam","subitem_relation_type_select":"URI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.ammcb.info/","subitem_relation_type_select":"URI"}}]},"item_4_rights_23":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright © 2009 by The Applied Microbiology, Molecular and Cellular Biosciences Research Foundation"}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA00698664","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0022-1260","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Mohamed Morsy, Fatthy"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kuzuha, Satomi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takani, Yayoi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sakamoto, Toshio"}],"nameIdentifiers":[{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-03"}],"displaytype":"detail","filename":"SC-PR-SAKAMOTO-T-243.pdf","filesize":[{"value":"606.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"SC-PR-SAKAMOTO-T-243.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/10636/files/SC-PR-SAKAMOTO-T-243.pdf"},"version_id":"8dfcc819-18bc-4d6d-970c-c936c3a40c5a"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Novel thermostable glycosidases in the extracellular matrix of the terrestrial cyanobacterium Nostoc commune","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Novel thermostable glycosidases in the extracellular matrix of the terrestrial cyanobacterium Nostoc commune"}]},"item_type_id":"4","owner":"3","path":["937"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-03"},"publish_date":"2017-10-03","publish_status":"0","recid":"10636","relation_version_is_last":true,"title":["Novel thermostable glycosidases in the extracellular matrix of the terrestrial cyanobacterium Nostoc commune"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-07-28T01:38:40.672973+00:00"}