WEKO3
インデックスリンク
アイテム
核酸感染系の特質
http://hdl.handle.net/2297/35697
http://hdl.handle.net/2297/35697f28ffbd4-1b80-4062-b6cf-53ce6f24b4f6
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
SC-PR-TAKETO-A-36.pdf (577.6 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2017-10-03 | |||||
| タイトル | ||||||
| タイトル | 核酸感染系の特質 | |||||
| タイトル | ||||||
| タイトル | Properties of Transfection Systems | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
武藤, 明
× 武藤, 明 |
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| 書誌情報 |
生物物理 = Biophysics 巻 17, 号 1, p. 36-44, 発行日 1977-01-01 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0582-4052 | |||||
| NCID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00129693 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.2142/biophys.17.36 | |||||
| 出版者 | ||||||
| 出版者 | 日本生物物理学会 = The Biophysical Society of Japan | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Properties of various transfection systems in E. coli have been surveyed. The recipient preparations include various spheroplasts (induced by lysozyme, penicillin, or glycine), plasmolyzed or osmotically-shocked cells, cell-envelope mutants, helper-infected bacteria, frozen-thawed cells and bacteria treated with Ca2+ or Ba2+. Efficiency of transfection depends on structure of nucleic acids as well as on competence of recipients. Thus, spheroplasts preferentially take up single-stranded nucleic acids, whereas Ca2+- or Ba2+-treated cells exhibit higher efficiency for double-stranded DNA. Unlike intact phages, free nucleic acids are sensitive to cellular nucleases even after uptake, and this sensitivity is also determined by structure of the infecting molecule (strandedness, circularity etc.). Mechanism of polynucleotide uptake into cytoplasm is quite obscure as yet. Although experimental evidence is lacking, it seems not improbable that nucleic acid penetrates a proteinous channel (or pore) rather than phospholipid layer. The Ca2+- or Ba2+-dependent uptake of DNA occurs very rapidly at low temperature and extremely sensitive to phosphate. Based on peculiar cryophilic property, an involvement of lipid crystallization has been postulated in this system. Such lipid crystallization might cause conformational change of protein pore to allow penetration of nucleic acid. Structure of the putative pore may be modulated by arrangement of lipopolysaccharides and other membrane proteins. Neutralization of negative charge on cell envelope is probably another effect of Ca2+ or Ba2+. | |||||
| 権利 | ||||||
| 権利情報 | Copyright © 2013 by THE BIOPHYSICAL SOCIETY OF JAPAN | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 関連URI | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.biophys.jp/ | |||||
| 関連URI | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://www.jstage.jst.go.jp/browse/biophys/-char/ja/ | |||||
