{"created":"2023-07-27T06:26:49.965876+00:00","id":10716,"links":{},"metadata":{"_buckets":{"deposit":"3d9c768f-d479-437e-807a-7299511e3758"},"_deposit":{"created_by":3,"id":"10716","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"10716"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00010716","sets":["934:935:937"]},"author_link":["11543","13493","16352","16350","16351"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-11-14","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"11","bibliographicPageStart":"e113156","bibliographicVolumeNumber":"9","bibliographic_titles":[{"bibliographic_title":"PLoS ONE"}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background: Silkmoth, Bombyx mori, is an ideal model insect for investigating the neural mechanisms underlying sex pheromone-induced innate behavior. Although transgenic techniques and the GAL4/UAS system are well established in the silkmoth, genetic tools useful for investigating brain function at the neural circuit level have been lacking. Results: In the present study, we established silkmoth strains in which we could visualize neural projections (UASmCD8GFP) and cell nucleus positions (UAS-GFP.nls), and manipulate neural excitability by thermal stimulation (UASdTrpA1). In these strains, neural projections and nucleus position were reliably labeled with green fluorescent protein in a GAL4-dependent manner. Further, the behavior of silkworm larvae and adults could be controlled by GAL4-dependent misexpression of dTrpA1. Ubiquitous dTrpA1 misexpression led both silkmoth larvae and adults to exhibit seizure-like phenotypes in a heat stimulation-dependent manner. Furthermore, dTrpA1 misexpression in the sex pheromone receptor neurons of male silkmoths allowed us to control male sexual behavior by changing the temperature. Thermally stimulated male silkmoths exhibited full sexual behavior, including wing-flapping, orientation, and attempted copulation, and precisely approached a thermal source in a manner similar to male silkmoths stimulated with the sex pheromone. Conclusion: These findings indicate that a thermogenetic approach using dTrpA1 is feasible in Lepidopteran insects and thermogenetic analysis of innate behavior is applicable in the silkmoth. These tools are essential for elucidating the relationships between neural circuits and function using neurogenetic methods.","subitem_description_type":"Abstract"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Public Library of Science"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1371/journal.pone.0113156","subitem_relation_type_select":"DOI"}}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1932-6203","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kiya, Taketoshi"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Morishita, Koudai"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Uchino, Keiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Iwami, Masafumi"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Sezutsu, Hideki"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-03"}],"displaytype":"detail","filename":"SC-PR-KIYA-T-113156.pdf","filesize":[{"value":"12.7 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"SC-PR-KIYA-T-113156.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/10716/files/SC-PR-KIYA-T-113156.pdf"},"version_id":"1462a01a-b6ac-4dcc-b173-27260297e98a"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Establishment of tools for neurogenetic analysis of sexual behavior in the silkmoth, Bombyx mori","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Establishment of tools for neurogenetic analysis of sexual behavior in the silkmoth, Bombyx mori"}]},"item_type_id":"4","owner":"3","path":["937"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-03"},"publish_date":"2017-10-03","publish_status":"0","recid":"10716","relation_version_is_last":true,"title":["Establishment of tools for neurogenetic analysis of sexual behavior in the silkmoth, Bombyx mori"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-07-28T01:37:26.937878+00:00"}