@article{oai:kanazawa-u.repo.nii.ac.jp:00010722, author = {福田, 真悟 and 内橋, 貴之 and 安藤, 敏夫 and Fukuda, Shingo and Uchihashi, Takayuki and Ando, Toshio}, issue = {6}, journal = {Review of Scientific Instruments}, month = {Jun}, note = {In tip-scan atomic force microscopy (AFM) that scans a cantilever chip in the three dimensions, the chip body is held on the Z-scanner with a holder. However, this holding is not easy for high-speed (HS) AFM because the holder that should have a small mass has to be able to clamp the cantilever chip firmly without deteriorating the Z-scanner's fast performance, and because repeated exchange of cantilever chips should not damage the Z-scanner. This is one of the reasons that tip-scan HS-AFM has not been established, despite its advantages over sample stage-scan HS-AFM. Here, we present a novel method of cantilever chip holding which meets all conditions required for tip-scan HS-AFM. The superior performance of this novel chip holding mechanism is demonstrated by imaging of the α3β3 subcomplex of F1-ATPase in dynamic action at ∼7 frames/s. © 2015 AIP Publishing LLC., 金沢大学ナノ生命科学研究所}, title = {Method of mechanical holding of cantilever chip for tip-scan high-speed atomic force microscope}, volume = {86}, year = {2015} }