{"created":"2023-07-27T06:27:01.388491+00:00","id":10992,"links":{},"metadata":{"_buckets":{"deposit":"3ced11bf-acdc-45dd-84d9-669406f10fff"},"_deposit":{"created_by":3,"id":"10992","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"10992"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00010992","sets":["934:935:937"]},"author_link":["16972","15083","16970","16974","16971","16973"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2003-06-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageEnd":"772","bibliographicPageStart":"767","bibliographicVolumeNumber":"133","bibliographic_titles":[{"bibliographic_title":"Journal of Biochemistry"}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Type III Cu ligand, His456 and His458, of Myrothecium verrucaria (MT-1) bilirubin oxidases (BO) [EC 1.3.3.5] were doubly mutated as to Lys, Asp, and Val. In spite of perturbation of the type III Cu centers, these mutants were pale blue or colourless when isolated. However, they became intense blue on reaction with reducing agents such as dithionite, ascorbate, hexacyanoferrate(II), and octacyanotangstate(IV) under air, or with an oxidizing agent such as hexacyanoferrate(III), indicating that they are in mixed forms when expressed in Aspergillus oryzae. His456.458Lys and His456.458Asp mutated as to potential coordinating groups showed weak BO and ferroxidase activities, while His 456.458Val mutated as to non-coordinating groups showed no enzyme activity at all.","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学理工研究域物質化学系","subitem_description_type":"Other"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本生化学会 = Japanese Biochemical Society"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1093/jb/mvg098","subitem_relation_type_select":"DOI"}}]},"item_4_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://jb.oxfordjournals.org/cgi/content/abstract/133/6/767","subitem_relation_type_select":"URI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1093/jb/mvg098","subitem_relation_type_select":"DOI"}}]},"item_4_rights_23":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2003 Japanese Biochemical Society"}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA00694073","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0021-924X","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Shimizu, Atsushi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Samejima, Tatsuya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hirota, Shun"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamaguchi, Shotaro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sakurai, Nobuhiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sakurai, Takeshi"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-03"}],"displaytype":"detail","filename":"SC-PR-SAKURAI-T-767.pdf","filesize":[{"value":"238.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"SC-PR-SAKURAI-T-767.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/10992/files/SC-PR-SAKURAI-T-767.pdf"},"version_id":"fa151684-8dce-4209-a54e-85a6fa2e0847"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Type III Cu mutants of Myrothecium verrucaria bilirubin oxidase","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Type III Cu mutants of Myrothecium verrucaria bilirubin oxidase"}]},"item_type_id":"4","owner":"3","path":["937"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-03"},"publish_date":"2017-10-03","publish_status":"0","recid":"10992","relation_version_is_last":true,"title":["Type III Cu mutants of Myrothecium verrucaria bilirubin oxidase"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-07-28T01:34:12.238239+00:00"}