{"created":"2023-07-27T06:28:25.634156+00:00","id":12983,"links":{},"metadata":{"_buckets":{"deposit":"d0ff50b5-42d0-42a0-b698-6b47c5088f1e"},"_deposit":{"created_by":3,"id":"12983","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"12983"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00012983","sets":["1132:1133:1134"]},"author_link":["20261","249","20260","182","92"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-10-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"8","bibliographicPageEnd":"942","bibliographicPageStart":"935","bibliographicVolumeNumber":"26","bibliographic_titles":[{"bibliographic_title":"Liver International"}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Background/Aim: Biliary epithelial cells possess an innate immune system consisting of Toll-like receptors (TLRs). Although the human bile contains lipopolysaccharide (LPS) in normal as well as diseased livers, LPS physiologically does not elicit an inflammatory response in the biliary tree. This absence of a response to LPS could be due to the 'endotoxin tolerance'speculated to maintain innate immune homeostasis in organs. Our aim here is to clarify the presence and molecular mechanisms of endotoxin tolerance of biliary epithelium. Methods and Results: In nuclear factor-κB (NF-κB)-DNA binding assays using three-cultured human intrahepatic biliary epithelial cell (HIBEC) lines, all the cells responded to LPS (TLR4 ligand) by activating NF-κB, but pretreatment with LPS for 24h effectively induced tolerance against any subsequent stimulation with LPS (endotoxin tolerance). This tolerance was also induced by pretreatment with Pam3Cys-Ser-(Lys)4 trihydrochloride (Pam3CKS4, TLR1/2 ligand). Then, real-time polymerase chain treaction and Western blotting revealed that LPS treatment upregulated the expression of IRAK-M (a negative regulator of TLR signaling), but did not affect interleukin-1 receptor-associated kinase-1 (IRAK-1, an essential molecule of TLR signaling), in HIBECs. Moreover, immunohistochemistry revealed that IRAK-M was diffusely expressed in intrahepatic bile ducts. Conclusions: This study showed that the mechanism of endotoxin tolerance exists in the intrahepatic biliary tree and is possibly induced by the expression of IRAK-M in the intrahepatic biliary epithelium, suggesting that the endotoxin tolerance is important in maintaining innate immune biliary homeostasis. © 2006 Blackwell Munksgaard.","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学大学院医学系研究科がん細胞学","subitem_description_type":"Other"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Blackwell Publishing"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1111/j.1478-3231.2006.01325.x","subitem_relation_type_select":"DOI"}}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA11817539","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1478-3223","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Harada, Kenichi"}],"nameIdentifiers":[{"nameIdentifier":"182","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"30283112","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=30283112"},{"nameIdentifier":"30283112","nameIdentifierScheme":"金沢大学研究者情報","nameIdentifierURI":"http://ridb.kanazawa-u.ac.jp/public/detail.php?kaken=30283112"},{"nameIdentifier":"30283112","nameIdentifierScheme":"研究者番号","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000030283112"}]},{"creatorNames":[{"creatorName":"Isse, Kumiko"}],"nameIdentifiers":[{"nameIdentifier":"20260","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sato, Yasunori"}],"nameIdentifiers":[{"nameIdentifier":"249","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"30324073","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=30324073"},{"nameIdentifier":"30324073","nameIdentifierScheme":"金沢大学研究者情報","nameIdentifierURI":"http://ridb.kanazawa-u.ac.jp/public/detail.php?kaken=30324073"},{"nameIdentifier":"30324073","nameIdentifierScheme":"研究者番号","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000030324073"}]},{"creatorNames":[{"creatorName":"Ozaki, Satoru"}],"nameIdentifiers":[{"nameIdentifier":"20261","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nakanuma, Yasuni"}],"nameIdentifiers":[{"nameIdentifier":"92","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"10115256","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=10115256"},{"nameIdentifier":"10115256","nameIdentifierScheme":"研究者番号","nameIdentifierURI":"https://nrid.nii.ac.jp/nrid/1000010115256"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-03"}],"displaytype":"detail","filename":"ME-PR-HARADA-K-935.pdf","filesize":[{"value":"2.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ME-PR-HARADA-K-935.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/12983/files/ME-PR-HARADA-K-935.pdf"},"version_id":"ffaf5640-b9ba-4c11-b208-4dc8a3b0e2c8"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Endotoxin tolerance in human intrahepatic biliary epithelial cells is induced by upregulation of IRAK-M","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Endotoxin tolerance in human intrahepatic biliary epithelial cells is induced by upregulation of IRAK-M"}]},"item_type_id":"4","owner":"3","path":["1134"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-03"},"publish_date":"2017-10-03","publish_status":"0","recid":"12983","relation_version_is_last":true,"title":["Endotoxin tolerance in human intrahepatic biliary epithelial cells is induced by upregulation of IRAK-M"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2024-06-20T06:57:37.737376+00:00"}