{"created":"2023-07-27T06:28:34.576991+00:00","id":13170,"links":{},"metadata":{"_buckets":{"deposit":"dd38892b-c6f0-4aef-8755-9e5c936c6844"},"_deposit":{"created_by":3,"id":"13170","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"13170"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00013170","sets":["1132:1133:1134"]},"author_link":["21031","21033","661","21032","21034"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2002-01-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"91","bibliographicPageStart":"84","bibliographicVolumeNumber":"272","bibliographic_titles":[{"bibliographic_title":"Experimental Cell Research"}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We found that the convergently epidermal growth factor (EGF)-induced signal and the collagen-induced signal activate mitogen-activated protein kinase (MAPK), which induces migration. We examined the signaling mechanisms of EGF-induced cell migration on collagen using the A431 carcinoma cell. EGF (10 ng/ml) induced migration on collagen, but inhibited proliferation. Using a MAPK cascade inhibitor, PD98059, it was shown that EGF-induced migration on collagen was mediated by MAPK whereas EGF-induced migration on fibronectin and vitronectin was not. PD98059 also showed that activation of MAPK induced by EGF enhanced the adhesiveness of A431 cells to collagen. By Western blotting analysis, the kinetics of MAPK phosphorylation induced by EGF and collagen was examined separately, and convergently. First of all, EGF without collagen caused transient MAPK phosphorylation. Collagen without EGF caused MAPK to be immediately and transiently dephosphorylated, and rephosphorylated followed by sustained hyperphosphorylation. EGF together with collagen caused an immediate, and sustained, hyperphosphorylation. These facts suggest that the transient MAPK dephosphorylation induced by collagen is required for migration in order to maintain an appropriate level of sustained phosphorylation. Furthermore, we found that adhesion of A431 cells to collagen was blocked by the anti-β1 integrin antibody or by the mixed antibodies composed of anti-α1, -α2, and -α3 antibodies, indicating that collagen-induced MAPK phosphorylation was mediated through α1β1, α2β1, and α3β1 integrins. © 2002 Elsevier Science.","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学大学院医学系研究科","subitem_description_type":"Other"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1006/excr.2001.5399","subitem_relation_type_select":"DOI"}}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0014-4827","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kawahara, Ei"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Nakada, Natsuko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hikichi, Tetsuro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kobayashi, Jun"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakanishi, Isao"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-03"}],"displaytype":"detail","filename":"HE-PR-KAWAHARA-S-001.pdf","filesize":[{"value":"233.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"HE-PR-KAWAHARA-S-001.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/13170/files/HE-PR-KAWAHARA-S-001.pdf"},"version_id":"f300d217-bff5-4baf-aeb1-491a19aac1ad"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"EGF and β1 Integrin Convergently Regulate Migration of A431 Carcinoma","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"EGF and β1 Integrin Convergently Regulate Migration of A431 Carcinoma"}]},"item_type_id":"4","owner":"3","path":["1134"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-03"},"publish_date":"2017-10-03","publish_status":"0","recid":"13170","relation_version_is_last":true,"title":["EGF and β1 Integrin Convergently Regulate Migration of A431 Carcinoma"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-07-27T11:35:12.105396+00:00"}