WEKO3
インデックスリンク
アイテム
Laboratory diagnosis of toxigenic Clostridium difficile by polymerase chain reaction: Presence of toxin genes and their stable expression in toxigenic isolates from Japanese individuals.
http://hdl.handle.net/2297/1869
http://hdl.handle.net/2297/1869563b6dda-4685-4bc8-b29f-28d24e55da07
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
HE-PR-KARASAWA-T-2.pdf (162.1 kB)
|
|
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2017-10-03 | |||||
| タイトル | ||||||
| タイトル | Laboratory diagnosis of toxigenic Clostridium difficile by polymerase chain reaction: Presence of toxin genes and their stable expression in toxigenic isolates from Japanese individuals. | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Karasawa, Tadahiro
× Karasawa, Tadahiro× Nojiri, Takashi× Hayashi, Yasuhiro× Maegawa, Tsuneo× Yamakawa, Kiyotaka× Wang, XingMin× Nakamura, Shinichi |
|||||
| 書誌情報 |
Journal of Gastroenterology 巻 34, 号 1, p. 41-45, 発行日 1999-02-01 |
|||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0944-1174 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1007/s005350050214 | |||||
| 出版者 | ||||||
| 出版者 | Springer Verlag | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Clostridium difficile causes pseudomembranous colitis and antibiotic-associated diarrhea. The definitive diagnosis of C. difficile infection is finally accomplished by the isolation of toxigenic C. difficile. However, only a small number of Japanese clinical laboratories are able to reach a definitive diagnosis of C. difficile infection, probably because simple reliable assays for toxins in the isolates are not available. In this study, we examined the compatibility of a polymerase chain reaction (PCR) assay and tissue culture assay to identify toxigenic C. difficile, in toxigenic and nontoxigenic C. difficile isolates from Japanese patients and healthy carriers. The specificity of PCR primers was demonstrated by restriction endonuclease digestion and seminested PCR in C. difficile VPI 10463 strain. No PCR product was amplified in the eight other clostridial species used to check the specifiecty of the PCR assay. The detection limit was 103 cells. Both toxin A and toxin B genes (the genes encoding the major virulence factors of C. difficile) were detected in 58 toxigenic C. difficile isolates, which showed a wide range of cytotoxic activity in tissue culture assays. Neither of the toxin genes was carried by 40 nontoxigenic strains of C. difficile. The results of this study strongly suggest that a definitive diagnosis of C. difficile infection can be accomplished by PCR detection of the toxin genes rather than by tissue culture assay of isolates. | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
