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Localization of actin and cytokeratin in hepatocytes and biliary epithelial cells in normal human livers using a low temperature resin and post-embedding immunohistochemistry
http://hdl.handle.net/2297/16864
http://hdl.handle.net/2297/1686471ebe897-cba4-42d6-a4dd-6ab0de084581
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ME0PR-NAKANUMA-Y-325.pdf (5.1 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2017-10-03 | |||||
| タイトル | ||||||
| タイトル | Localization of actin and cytokeratin in hepatocytes and biliary epithelial cells in normal human livers using a low temperature resin and post-embedding immunohistochemistry | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Kono, Naoko
× Kono, Naoko× Nakanuma, Yasuni |
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| 提供者所属 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 金沢大学医薬保健研究域医学系 | |||||
| 書誌情報 |
Tohoku Journal of Experimental Medicine 巻 157, 号 4, p. 325-335, 発行日 1989-01-01 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0040-8727 | |||||
| NCID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00863920 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1620/tjem.157.325 | |||||
| 出版者 | ||||||
| 出版者 | 東北大学 : 東北ジャーナル刊行会 | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Using a low temperature resin Lowicryl K4M and post-embedding immunogold labeling, localization of actin and cytokeratin was examined in hepatocytes and biliary epithelial cells in normal human livers. Preservation of the liver tissues was satisfactory at the electron microscopic level. In immunolabeling of actin, gold particles were mainly found on microvilli and around the junctional complexes and at cell borders in the hepatocytes as well as biliary epithelial cells. In immunolabeling of cytokeratin, the gold particles were rather preferentially found in the perinuclear cytoplasm and around the junctional complexes in the biliary epithelial cells and hepatocytes. These particles were mainly found to be located on the intermediate filaments at higher magnification, although a considerable number of intermediate filaments were not labeled in the hepatocytes and biliary epithelial cells. Double staining method using the gold particles of different sizes clearly confirmed the abovementioned distribution of actin and cytokeratin in the same cells. From these observations, it was suggested that post-embedding immunoelectron microscopy using Lowicryl K4M is a useful tool for analysis of cytoskeletal organization in the liver tissue. | |||||
| 権利 | ||||||
| 権利情報 | Copyright© Tohoku University Medical Press | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 関連URI | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://journal.med.tohoku.ac.jp/ | |||||
| 関連URI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | http://dx.doi.org/10.1620/tjem.157.325 | |||||
| 関連URI | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.journalarchive.jst.go.jp/jnlpdf.php?cdjournal=tjem1920&cdvol=157&noissue=4&startpage=325&lang=en&from=jnlabstract | |||||
