{"created":"2023-07-27T06:28:48.637746+00:00","id":13383,"links":{},"metadata":{"_buckets":{"deposit":"cb582be6-70d2-4ea9-8859-988fb91a64cb"},"_deposit":{"created_by":3,"id":"13383","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"13383"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00013383","sets":["1132:1133:1134"]},"author_link":["1004","21877","21878","62","1094","49","21876","337"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-12-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"12","bibliographicPageEnd":"1013","bibliographicPageStart":"1002","bibliographicVolumeNumber":"12","bibliographic_titles":[{"bibliographic_title":"Journal of Gene Medicine"}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The prognosis of patients with hepatocellular carcinoma (HCC) remains poor, largely as a result of intrahepatic metastasis. Using a mouse model of intrahepatic metastasis, we investigated whether chemokine ligand 2/monocyte chemoattractant protein-1 (CCL2/MCP-1) could potentiate the antitumor effects of the herpes simplex virus thymidine kinase/ganciclovir (HSV-tk/GCV) system.Methods: Mouse hepatoma cells infected with recombinant adenovirus vectors expressing HSV-tk, CCL2/MCP-1 and LacZ at multiplicities of infection of Ad-tk/Ad-MCP1 = 3/0.03 (T/MLow), 3/3 (T/MHigh) and Ad-tk/Ad-LacZ = 3/3 (T/L) were injected into BALB/c mice.Results: Intrahepatic tumor growth was significantly lower in T/MLow mice. By contrast, no tumor suppression was observed in T/MHigh mice. The tumor-specific cytolytic activities of splenocytes from T/MLow and T/MHigh mice were comparable. Immunohistochemical analysis of liver tissues showed similar infiltration by Mac-1+ and T cells in these animals, whereas the proportions of classical activated (M1) monocytes/macrophages were significantly higher in T/MLow mice. In addition, interleukin-12 production was elevated in these tissues. Vascular endothelial growth factor-A expression and CD31+ microvessels were increased in T/MHigh mice.Conclusions: Collectively, these results demonstrate that an adequate amount of CCL2/MCP-1, together with the HSV-tk/GCV system, may induce T helper 1-polarized antitumor effects without inducing tumor angiogenesis in the microenvironment of intrahepatic HCC progression. © 2010 John Wiley & Sons, Ltd.","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医薬保健研究域医学系","subitem_description_type":"Other"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Wiley-Blackwell"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1002/jgm.1528","subitem_relation_type_select":"DOI"}}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA11470125","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1099-498X","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kakinoki, Kaheita"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Nakamoto, Yasunari"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kagaya, Takashi"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Tsuchiyama, Tomoya"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Sakai, Yoshio"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Nakahama, Tohru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mukaida, Naofumi"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Kaneko, Shuichi"}],"nameIdentifiers":[{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-03"}],"displaytype":"detail","filename":"ME-PR-KANEKO-S-1002.pdf","filesize":[{"value":"1.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ME-PR-KANEKO-S-1002.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/13383/files/ME-PR-KANEKO-S-1002.pdf"},"version_id":"ad57a322-5df9-470e-bb25-6aa1347c0919"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Prevention of intrahepatic metastasis of liver cancer by suicide gene therapy and chemokine ligand 2/monocyte chemoattractant protein-1 delivery in mice","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Prevention of intrahepatic metastasis of liver cancer by suicide gene therapy and chemokine ligand 2/monocyte chemoattractant protein-1 delivery in mice"}]},"item_type_id":"4","owner":"3","path":["1134"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-03"},"publish_date":"2017-10-03","publish_status":"0","recid":"13383","relation_version_is_last":true,"title":["Prevention of intrahepatic metastasis of liver cancer by suicide gene therapy and chemokine ligand 2/monocyte chemoattractant protein-1 delivery in mice"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-07-28T01:06:04.544387+00:00"}