@article{oai:kanazawa-u.repo.nii.ac.jp:00013637,
 author = {中河, 秀俊 and 水腰, 英四郎 and 飯田, 宗穂 and 寺島, 健志 and 北原, 征明 and 北村, 和哉 and 中本, 安成 and 金子, 周一 and Nakagawa, Hidetoshi and Mizukoshi, Eishiro and Iida, Noriho and Terashima, Takeshi and Kitahara, Masaaki and Marukawa, Yohei and Kitamura, Kazuya and Nakamoto, Yasunari and Hiroishi, Kazumasa and Imawari, Michio and Kaneko, Shuichi},
 issue = {4},
 journal = {Cancer Immunology, Immunotherapy},
 month = {Apr},
 note = {Radiofrequency ablation therapy (RFA) is a radical treatment for liver cancers and induces tumor antigen-specific immune responses. In the present study, we examined the antitumor effects of focal OK-432-stimulated dendritic cell (DC) transfer combined with RFA and analyzed the functional mechanisms involved using a murine model. C57BL/6 mice were injected subcutaneously with colon cancer cells (MC38) in their bilateral flanks. After the establishment of tumors, the subcutaneous tumor on one flank was treated using RFA, and then OK-432-stimulated DCs were injected locally. The antitumor effect of the treatment was evaluated by measuring the size of the tumor on the opposite flank, and the immunological responses were assessed using tumor-infiltrating lymphocytes, splenocytes and draining lymph nodes. Tumor growth was strongly inhibited in mice that exhibited efficient DC migration after RFA and OK-432-stimulated DC transfer, as compared to mice treated with RFA alone or treatment involving immature DC transfer. We also demonstrated that the antitumor effect of this treatment depended on both CD8-positive and CD4-positive cells. On the basis of our findings, we believe that combination therapy for metastatic liver cancer consisting of OK-432-stimulated DCs in combination with RFA can proceed to clinical trials, and it is anticipated to be markedly superior to RFA single therapy. © 2013 Springer-Verlag Berlin Heidelberg., 金沢大学先進予防医学研究センター / 金沢大学医薬保健研究域医学系},
 pages = {347--356},
 title = {In vivo immunological antitumor effect of OK-432-stimulated dendritic cell transfer after radiofrequency ablation},
 volume = {63},
 year = {2014}
}