{"created":"2023-07-27T06:29:00.532157+00:00","id":13648,"links":{},"metadata":{"_buckets":{"deposit":"cea25921-2d28-48bd-a6ac-793332464209"},"_deposit":{"created_by":3,"id":"13648","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"13648"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00013648","sets":["1132:1133:1134"]},"author_link":["22780","1150","22776","22777","223","22781","22779","22778"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-03-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"79","bibliographicPageStart":"73","bibliographicVolumeNumber":"10","bibliographic_titles":[{"bibliographic_title":"形態・機能 = Structure and function"}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"（目的）先の研究において、キトサンオリゴ糖を腹膜腔に長期間投与すると腹膜炎が 惹起され、横隔膜に細胞塊が形成されることを明らかにした。今回は、キトサンオリ ゴ糖よりも低分子の単糖であるブドウ糖、二糖であるショ糖を腹膜腔に投与し、腹水 が貯留するか、および横隔膜に細胞塊が形成されるかを観察した。（方法）C57BL/6 雄マウスの腹膜腔内に0.1％のキトサンオリゴ糖・ブドウ糖・ショ糖それぞれ0.2ml を1 日に1 回、14 日間投与した。14 日間後に安楽死させ、腹膜腔を観察し、かつ、 横隔膜の細胞塊を採取して電子顕微鏡で観察した。（結果と考察）先の研究と同様に キトサンオリゴ糖投与のマウスには大量の乳白色の腹水が観察され、白色の細胞塊が 主に横隔膜の腹膜面に観察された。一方、ブドウ糖、ショ糖を投与したマウスに、大 量の乳白色の腹水や白色の細胞塊は観察されなかった。白色の細胞塊は，横隔膜の腹 膜下においてリンパ管が発達している部位に見られた。細胞塊はキトサンオリゴ糖を 貪食した多数の炎症細胞からなり、細胞間にコラーゲンおよび血管も見られた。この 細胞塊は乳斑に類似していた。中皮細胞間からコラーゲンが露出し、リンパ管小孔と 思われる部位を通過する細胞も観察された。キトサンオリゴ糖を貪食する大食細胞が 見られ、このような細胞は拡張したリンパ管内にも観察された。以上の結果から、キ トサンオリゴ糖は乳斑の細胞に貪食され、さらにリンパ管小孔からリンパ管に取り込 まれてリンパ管内においても細胞に貪食され、炎症反応が誘起されたと考えられた。Aim: Peritonitis was evoked when we administered chitooligosaccharide into the peritoneal cavity in a former long-term study. We clarified that a cell mass was formed on the diaphragm. In this study we observed the cell masses on the diaphragm using an electron microscope and we aimed to determine whether the peritonitis occurred after administration of glucose (monosaccharide) and sucrose (disaccharide), which have lower molecular weights than chitooligosaccharide. into the peritoneal cavity. Methods: We administered a 0.2 mL solution containing 0.1% chitooligosaccharide, glucose, or sucrose into the peritoneal cavity of C57BL/6 male mice once a day for 14 days. After a 14-day observation period we euthanized the mice, observed the peritoneal cavities, gathered the cell masses, and observed them with an electron microscope. Results: A large quantity of white ascites was present in the peritoneal cavity and the white masses were observed on the diaphragm of the mice treated with chitooligosaccharide. These masses were not observed in mice treated with glucose or sucrose. The white cell masses formed on the peritoneal area where the lymphatic vessel under the diaphragmatic serosa developed and the lymphatic stomata opened. The mass was comprised of a large number of inflammatory cells, with collagen fibers among the cells. Blood vessels also were observed in the mass. Therefore, the masses appeared to be the milky spot. Collagen was exposed from the intercellular space between mesothelial cells. Cells, which travelled through the lymphatic stomata, were observed. Cells that had phagocytized chitooligosaccharide were observed in enlarged lymphatics. Conclusion: Considered from these findings, it was suggested that chitooligosaccharide was phagocytized by cells in the milky spot and the lymphatics, evoking an inflammatory reaction.","subitem_description_type":"Abstract"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"コ・メディカル形態機能学研究会"}]},"item_4_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://square.umin.ac.jp/co-medsf/index.html","subitem_relation_type_select":"URI"}}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA12137631","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1347-7145","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"向井, 加奈恵"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"浅野, きみ"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"中島, 由加里"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"高田, 佳奈"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"原, 由里子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"浦井, 珠恵"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"松尾, 淳子"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"中谷, 壽男"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-03"}],"displaytype":"detail","filename":"ME-PR-NAKATANI-T-73.pdf","filesize":[{"value":"3.5 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ME-PR-NAKATANI-T-73.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/13648/files/ME-PR-NAKATANI-T-73.pdf"},"version_id":"f3cb4f94-408c-4b5d-a2ce-da936a0a0c0b"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"キトサンオリゴ糖の腹膜腔内投与により誘発される腹膜炎の組織像","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"キトサンオリゴ糖の腹膜腔内投与により誘発される腹膜炎の組織像"},{"subitem_title":"Histological features of peritonitis induced by intraperitoneal injection of chitooligosaccharide","subitem_title_language":"en"}]},"item_type_id":"4","owner":"3","path":["1134"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-03"},"publish_date":"2017-10-03","publish_status":"0","recid":"13648","relation_version_is_last":true,"title":["キトサンオリゴ糖の腹膜腔内投与により誘発される腹膜炎の組織像"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-07-28T01:00:57.430163+00:00"}