{"created":"2023-07-27T06:29:02.066622+00:00","id":13684,"links":{},"metadata":{"_buckets":{"deposit":"d5bddf86-4475-4f3b-a692-a6b26d596f22"},"_deposit":{"created_by":3,"id":"13684","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"13684"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00013684","sets":["1132:1133:1134"]},"author_link":["1004","22890","22893","62","22891","108","22892","1094","337","49"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-05-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"319","bibliographicPageStart":"312","bibliographicVolumeNumber":"19","bibliographic_titles":[{"bibliographic_title":"Cancer Gene Therapy"}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Suicide gene therapy using the herpes simplex virus thymidine kinase/ganciclovir (HSV-tk/GCV) system combined with monocyte chemoattractant protein-1 (MCP-1) provides significant antitumor efficacy. The current study was designed to evaluate the antitumor immunity of a newly developed membrane-bound form of MCP-1 (mMCP-1) in an immunocompetent mouse model of hepatocellular carcinoma (HCC). A recombinant adenovirus vector (rAd) harboring the human MCP-1 gene and the membrane-spanning domain of the CX3CL1 gene was used. Large amounts of MCP-1 protein were expressed and accumulated on the tumor cell surface. The growth of subcutaneous tumors was markedly suppressed when tumors were treated with mMCP-1, as compared with soluble MCP-1, in combination with the HSV-tk/GCV system (P<0.01). The numbers of Mac-1-, CD4-and CD8a-positive cells were significantly higher in tumor tissues (P<0.05), and tumor necrosis factor (TNF) mRNA expression levels with mMCP-1 were almost five-fold higher than those with soluble MCP-1. These results indicate that the delivery of the mMCP-1 gene greatly enhanced antitumor effects following the apoptotic stimuli by promoting the recruitment and activation of macrophages and T lymphocytes, suggesting a novel strategy of immune-based gene therapy in the treatment of patients with HCC. © 2012 Macmillan Publishers Limited All rights reserved.","subitem_description_type":"Abstract"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Nature Publishing Group"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1038/cgt.2012.3","subitem_relation_type_select":"DOI"}}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA11052453","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0929-1903","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Marukawa, Yohei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakamoto, Yasunari"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Kakinoki, Kaheita"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Tsuchiyama, Tomoya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Iida, Noriho"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kagaya, Takashi"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Sakai, Yoshio"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Naito, Makoto"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mukaida, Naofumi"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Kaneko, Shuichi"}],"nameIdentifiers":[{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-03"}],"displaytype":"detail","filename":"ME-PR-KANEKO-S-312.pdf","filesize":[{"value":"520.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ME-PR-KANEKO-S-312.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/13684/files/ME-PR-KANEKO-S-312.pdf"},"version_id":"59a3122c-2a83-4005-a0df-4ad3159cf292"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Membrane-bound form of monocyte chemoattractant protein-1 enhances antitumor effects of suicide gene therapy in a model of hepatocellular carcinoma","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Membrane-bound form of monocyte chemoattractant protein-1 enhances antitumor effects of suicide gene therapy in a model of hepatocellular carcinoma"}]},"item_type_id":"4","owner":"3","path":["1134"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-03"},"publish_date":"2017-10-03","publish_status":"0","recid":"13684","relation_version_is_last":true,"title":["Membrane-bound form of monocyte chemoattractant protein-1 enhances antitumor effects of suicide gene therapy in a model of hepatocellular carcinoma"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-07-28T01:00:19.898637+00:00"}