@article{oai:kanazawa-u.repo.nii.ac.jp:00013830,
 author = {杉谷, 加代 and 大貝, 和裕 and 郡山, 恵樹 and 谷井, 秀治 and 松川, 通 and 加藤, 聖 and Sugitani, Kayo and Ogai, Kazuhiro and Hitomi, Kiyotaka and Nakamura-Yonehara, Kayo and Shintani, Takafumi and Noda, Masaharu and Koriyama, Yoshiki and Tanii, Hideji and Matsukawa, Toru and Kato, Satoru},
 issue = {3},
 journal = {Neurochemistry International},
 month = {Aug},
 note = {Unlike in mammals, fish retinal ganglion cells (RGCs) have a capacity to repair their axons even after optic nerve transection. In our previous study, we isolated a tissue type transglutaminase (TG) from axotomized goldfish retina. The levels of retinal TG (TG R) mRNA increased in RGCs 1-6 weeks after nerve injury to promote optic nerve regeneration both in vitro and in vivo. In the present study, we screened other types of TG using specific FITC-labeled substrate peptides to elucidate the implications for optic nerve regeneration. This screening showed that the activity of only cellular coagulation factor XIII (cFXIII) was increased in goldfish optic nerves just after nerve injury. We therefore cloned a full-length cDNA clone of FXIII A subunit (FXIII-A) and studied temporal changes of FXIII-A expression in goldfish optic nerve and retina during regeneration. FXIII-A mRNA was initially detected at the crush site of the optic nerve 1 h after injury; it was further observed in the optic nerve and achieved sustained long-term expression (1-40 days after nerve injury). The cells producing FXIII-A were astrocytes/microglial cells in the optic nerve. By contrast, the expression of FXIII-A mRNA and protein was upregulated in RGCs for a shorter time (3-10 days after nerve injury). Overexpression of FXIII-A in RGCs achieved by lipofection induced significant neurite outgrowth from unprimed retina, but not from primed retina with pretreatment of nerve injury. Addition of extracts of optic nerves with injury induced significant neurite outgrowth from primed retina, but not from unprimed retina without pretreatment of nerve injury. The transient increase of cFXIII in RGCs promotes neurite sprouting from injured RGCs, whereas the sustained increase of cFXIII in optic nerves facilitates neurite elongation from regrowing axons. © 2012 Elsevier Ltd. All rights reserved.},
 pages = {423--432},
 title = {A distinct effect of transient and sustained upregulation of cellular factor XIII in the goldfish retina and optic nerve on optic nerve regeneration},
 volume = {61},
 year = {2012}
}