{"created":"2023-07-27T06:29:11.721731+00:00","id":13911,"links":{},"metadata":{"_buckets":{"deposit":"c98aca4d-957a-4901-8a61-d43bedea513f"},"_deposit":{"created_by":3,"id":"13911","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"13911"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00013911","sets":["4281:4291:4312"]},"author_link":["23517","371","72","2865","433","680","332","23291"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-12-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"7","bibliographicPageEnd":"1211","bibliographicPageStart":"1202","bibliographicVolumeNumber":"61","bibliographic_titles":[{"bibliographic_title":"Neurochemistry International"}]}]},"item_4_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"大貝, 和裕"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"馬渡, 一浩"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"杉谷, 加代"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"郡山, 恵樹"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"中島, 広志"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"加藤, 聖"}],"nameIdentifiers":[{},{},{}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Unlike mammals, fish motor function can recover within 6-8 weeks after spinal cord injury (SCI). The motor function of zebrafish is regulated by dual control; the upper motor neurons of the brainstem and motor neurons of the spinal cord. In this study, we aimed to investigate the framework behind the regeneration of upper motor neurons in adult zebrafish after SCI. In particular, we investigated the cell survival of axotomized upper motor neurons and its molecular machinery in zebrafish brain. As representative nuclei of upper motor neurons, we retrogradely labeled neurons in the nucleus of medial longitudinal fasciculus (NMLF) and the intermediate reticular formation (IMRF) using a tracer injected into the lesion site of the spinal cord. Four to eight neurons in each thin sections of the area of NMLF and IMRF were successfully traced at least 1-15 days after SCI. TUNEL staining and BrdU labeling assay revealed that there was no apoptosis or cell proliferation in the axotomized neurons of the brainstem at various time points after SCI. In contrast, axotomized neurons labeled with a neurotracer showed increased expression of anti-apoptotic factors, such as Bcl-2 and phospho-Akt (p-Akt), at 1-6 days after SCI. Such a rapid increase of Bcl-2 and p-Akt protein levels after SCI was quantitatively confirmed by western blot analysis. These data strongly indicate that upper motor neurons in the NMLF and IMRF can survive and regrow their axons into the spinal cord through the rapid activation of anti-apoptotic molecules after SCI. The regrowing axons from upper motor neurons reached the lesion site at 10-15 days and then crossed at 4-6 weeks after SCI. These long-distance descending axons from originally axotomized neurons have a major role in restoration of motor function after SCI. © 2012 Elsevier Ltd. All rights reserved.","subitem_description_type":"Abstract"}]},"item_4_description_22":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Thesis of Kazuhiro Ogai / 大貝 和裕 博士論文 金沢大学医薬保健学総合研究科（保健学専攻）","subitem_description_type":"Other"}]},"item_4_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00013898","subitem_identifier_reg_type":"JaLC"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.neuint.2012.08.015","subitem_relation_type_select":"DOI"}}]},"item_4_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.elsevier.com/locate/issn/01970186","subitem_relation_type_select":"URI"}}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA0032399X","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0197-0186","subitem_source_identifier_type":"ISSN"}]},"item_4_text_2":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_text_value":"ゼブラフィッシュ脊髄損傷後の上位運動ニューロンにおける抗アポトーシス因子の発現上昇について"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ogai, Kazuhiro"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Hisano, Suguru"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Mawatari, Kazuhiro"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Sugitani, Kayo"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Koriyama, Yoshiki"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Nakashima, Hiroshi"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Kato, Satoru"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-03"}],"displaytype":"detail","filename":"ME-PR-OGAI-K-1202.pdf","filesize":[{"value":"1.7 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-OGAI-K-1202.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/13911/files/ME-PR-OGAI-K-1202.pdf"},"version_id":"3d709219-0330-4a8b-891b-eb1112c26a0a"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Upregulation of anti-apoptotic factors in upper motor neurons after spinal cord injury in adult zebrafish","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Upregulation of anti-apoptotic factors in upper motor neurons after spinal cord injury in adult zebrafish"}]},"item_type_id":"4","owner":"3","path":["4312"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-03"},"publish_date":"2017-10-03","publish_status":"0","recid":"13911","relation_version_is_last":true,"title":["Upregulation of anti-apoptotic factors in upper motor neurons after spinal cord injury in adult zebrafish"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-07-27T08:51:06.263379+00:00"}