{"created":"2023-07-27T06:29:11.993656+00:00","id":13917,"links":{},"metadata":{"_buckets":{"deposit":"52d177bc-9e0d-45cb-a57d-1aab06264183"},"_deposit":{"created_by":3,"id":"13917","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"13917"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00013917","sets":["4281:4291:4312"]},"author_link":["371","22615","72","2865","2604","23533","332","23532","23291"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-12-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"7","bibliographicPageEnd":"1253","bibliographicPageStart":"1242","bibliographicVolumeNumber":"61","bibliographic_titles":[{"bibliographic_title":"Neurochemistry International"}]}]},"item_4_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"郡山, 恵樹"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"荒井, 國三"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"杉谷, 加代"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"大貝, 和裕"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"加藤, 聖"}],"nameIdentifiers":[{},{},{}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Nipradilol (Nip), which has α1- and β-adrenoceptor antagonist and nitric oxide (NO)-donating properties, has clinically been used as an anti-glaucomatous agent in Japan. NO mediates cellular signaling pathways that regulate physiological functions. The major signaling mechanisms mediated by NO are cGMP-dependent signaling and protein S-nitrosylation-dependent signalings. Nip has been described as having neuroprotective effects through cGMP-dependent pathway in retinal ganglion cells (RGCs). However, the effect seems to be partial. On the other hand, whether Nip can prevent cell death through S-nitrosylation is not yet clarified. In this study, we therefore focused on the neuroprotective mechanism of Nip through S-nitrosylation. Nip showed a dramatic neuroprotective effect against oxidative stress-induced death of RGC-5 cells. However, denitro-nipradilol, which does not have NO-donating properties, was not protective against oxidative stress. Furthermore, an NO scavenger significantly reversed the protective action of Nip against oxidative stress. In addition, we demonstrated that α1- or β-adrenoceptor antagonists (prazosin or timolol) did not show any neuroprotective effect against oxidative stress in RGC-5 cells. We also demonstrated that Nip induced the expression of the NO-dependent antioxidant enzyme, heme oxygenase-1 (HO-1). S-nitrosylation of Kelch-like ECH-associated protein by Nip was shown to contribute to the translocation of NF-E2-related factor 2 to the nucleus, and triggered transcriptional activation of HO-1. Furthermore, RGC death and levels of 4-hydroxy-2-nonenal (4HNE) were increased after optic nerve injury in vivo. Pretreatment with Nip significantly suppressed RGC death and accumulation of 4HNE after injury through an HO-1 activity-dependent mechanism. These data demonstrate a novel neuroprotective action of Nip against oxidative stress-induced RGC death in vitro and in vivo. © 2012 Elsevier Ltd. All rights reserved.","subitem_description_type":"Abstract"}]},"item_4_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00013904","subitem_identifier_reg_type":"JaLC"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.neuint.2012.09.004","subitem_relation_type_select":"DOI"}}]},"item_4_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.elsevier.com/locate/issn/01970186","subitem_relation_type_select":"URI"}}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA0032399X","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0197-0186","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Koriyama, Yoshiki"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Kamiya, Marie"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takadera, Tsuneo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Arai, Kunizo"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Sugitani, Kayo"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Ogai, Kazuhiro"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Kato, Satoru"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-03"}],"displaytype":"detail","filename":"ME-PR-KORIYAMA-Y-1242.pdf","filesize":[{"value":"13.2 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"ME-PR-KORIYAMA-Y-1242.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/13917/files/ME-PR-KORIYAMA-Y-1242.pdf"},"version_id":"8804a4f1-f588-4520-94ad-5f84a46be85c"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Protective action of nipradilol mediated through S-nitrosylation of Keap1 and HO-1 induction in retinal ganglion cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Protective action of nipradilol mediated through S-nitrosylation of Keap1 and HO-1 induction in retinal ganglion cells"}]},"item_type_id":"4","owner":"3","path":["4312"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-03"},"publish_date":"2017-10-03","publish_status":"0","recid":"13917","relation_version_is_last":true,"title":["Protective action of nipradilol mediated through S-nitrosylation of Keap1 and HO-1 induction in retinal ganglion cells"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-07-27T08:51:05.388427+00:00"}