{"created":"2023-07-27T06:29:15.149276+00:00","id":13990,"links":{},"metadata":{"_buckets":{"deposit":"6853cef2-147b-497f-a629-8bb1a2b22811"},"_deposit":{"created_by":3,"id":"13990","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"13990"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00013990","sets":["1132:1133:1134"]},"author_link":["23704","21890","23703","23706","23709","23707","465","23705","23708"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-01-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"21","bibliographicPageStart":"9","bibliographicVolumeNumber":"1512","bibliographic_titles":[{"bibliographic_title":"Brain Research"}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Signaling pathways involving phospholipase C (PLC) are involved in various neural functions. Understanding how these pathways are regulated will lead to a better understanding of their roles in neural functions. Previous studies demonstrated that receptor-driven PLCβ activation depends on intracellular Ca2+ concentration ([Ca2+]i), suggesting the possibility that PLCβ-dependent cellular responses are basically Ca 2+ dependent. To test this possibility, we examined whether modulations of ion channels driven by PLC-coupled metabotropic receptors are sensitive to [Ca2+]i using cultured hippocampal neurons. Muscarinic activation triggered an inward current at -100 mV (the equilibrium potential for K+) in a subpopulation of neurons. This current response was suppressed by pirenzepine (an M1-preferring antagonist), PLC inhibitor, non-selective cation channel blocker, and lowering [Ca 2+]i. Using the neurons showing no response at -100 mV, effects of muscarinic activation on K+ channels were examined at -40 mV. Muscarinic activation induced a transient decrease of the holding outward current. This current response was mimicked and occluded by XE991, an M-current K+ channel blocker, suppressed by pirenzepine, PLC inhibitor and lowering [Ca2+]i, and enhanced by elevating [Ca 2+]i. Similar results were obtained when group I metabotropic glutamate receptors were activated instead of muscarinic receptors. These results clearly show that ion channel modulations driven by PLC-coupled metabotropic receptors are dependent on [Ca2+]i, supporting the hypothesis that cellular responses induced by receptor-driven PLCβ activation are basically Ca2+ dependent. © 2013 Elsevier B.V.","subitem_description_type":"Abstract"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier B. V."}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.brainres.2013.03.040","subitem_relation_type_select":"DOI"}}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA1152177X","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0006-8993","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sugawara, Yuto"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Echigo, Ryousuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kashima, Kousuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Minami, Hanae"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Watanabe, Megumi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nishikawa, Yuiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Muranishi, Miho"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoneda, Mitsugu"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Ohno-Shosaku, Takako"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-03"}],"displaytype":"detail","filename":"ME-PR-SHOSAKU-T-9.pdf","filesize":[{"value":"331.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ME-PR-SHOSAKU-T-9.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/13990/files/ME-PR-SHOSAKU-T-9.pdf"},"version_id":"ffbc8ba2-6daf-45d1-9054-8ecbdb073894"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Intracellular calcium level is an important factor influencing ion channel modulations by PLC-coupled metabotropic receptors in hippocampal neurons","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Intracellular calcium level is an important factor influencing ion channel modulations by PLC-coupled metabotropic receptors in hippocampal neurons"}]},"item_type_id":"4","owner":"3","path":["1134"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-03"},"publish_date":"2017-10-03","publish_status":"0","recid":"13990","relation_version_is_last":true,"title":["Intracellular calcium level is an important factor influencing ion channel modulations by PLC-coupled metabotropic receptors in hippocampal neurons"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-07-28T00:55:03.694067+00:00"}