@article{oai:kanazawa-u.repo.nii.ac.jp:00014084,
 author = {本多, 政夫 and 山下, 太郎 and 白崎, 尚芳 and 西川, 昌志 and 丹尾, 幸樹 and 荒井, 邦明 and 酒井, 佳夫 and 山下, 竜也 and 水腰, 英四郎 and 金子, 周一 and Kawaguchi, Kazunori and Honda, Masao and Yamashita, Taro and Okada, Hikari and Shirasaki, Takayoshi and Nishikawa, Masashi and Nio, Kouki and Arai, Kuniaki and Sakai, Yoshio and Yamashita, Tatsuya and Mizukoshi, Eishiro and Kaneko, Shuichi},
 issue = {8},
 journal = {American Journal of Pathology},
 month = {Aug},
 note = {Notch signaling abnormalities are reported to be involved in the acceleration of malignancy in solid tumors and stem cell formation or regeneration in various organs. We analyzed specific genes for DNA copy number variations in liver cancer cells and investigated whether these factors relate to clinical outcome. Chromosome 20p, which includes the ligand for Notch pathways, Jagged1, was found to be amplified in several types of hepatoma cells, and its mRNA was up-regulated according to α-fetoprotein gene expression levels. Notch inhibition using Jagged1 shRNA and γ-secretase inhibitors produced significant suppression of cell growth in α-fetoprotein–producing cells with suppression of downstream genes. Using in vivo hepatoma models, the administration of γ-secretase inhibitors resulted in reduced tumor sizes and effective Notch inhibition with widespread apoptosis and necrosis of viable tumor cells. The γ-secretase inhibitors suppressed cell growth of the epithelial cell adhesion molecule–positive fraction in hepatoma cells, indicating that Notch inhibitors could suppress the stem cell features of liver cancer cells. Even in clinical liver cancer samples, the expression of α-fetoprotein and Jagged1 showed significant correlation, and amplification of the copy number of Jagged1 was associated with Jagged1 mRNA expression and poor survival after liver cancer surgical resection. In conclusion, amplification of Jagged1 contributed to mRNA expression that activates the Jagged1-Notch signaling pathway in liver cancer and led to poor outcome. © 2016 American Society for Investigative Pathology, Embargo Period 12 months, 金沢大学先進予防医学研究センター / 金沢大学医薬保健研究域医学系},
 pages = {2055--2067},
 title = {Jagged1 DNA Copy Number Variation Is Associated with Poor Outcome in Liver Cancer},
 volume = {186},
 year = {2016}
}