@book{oai:kanazawa-u.repo.nii.ac.jp:00014191,
 author = {Mieda, Michihiro and Sakurai, Takeshi},
 issue = {the series Neuromethods},
 month = {Sep},
 note = {Sleep and wakefulness are controlled by a complex network of neurons harboring diverse neurochemical characteristics, including glutamatergic, GABAergic, monoaminergic, cholinergic, and peptidergic neurons. To understand the precise role of each type of neuron in this circuit, it is useful to artificially manipulate the activity of a particular type of neuron to see its effect on behavior. The DREADD system has made such a strategy possible. Here, we review our recent work using DREADD to pharmacogenetically dissect neural mechanisms regulating sleep-wakefulness, describe the protocol we used, and discuss the technical aspects of our studies. © Springer Science+Business Media New York 2015. All rights reserved., Book Chapter, Embargo Period 12 months},
 publisher = {Springer Science+Business Media},
 title = {Pharmacogenetic dissection of neural mechanisms underlying the regulation of sleep-wakefulness using DREADDs},
 volume = {108},
 year = {2015}
}