{"created":"2023-07-27T06:29:25.938881+00:00","id":14221,"links":{},"metadata":{"_buckets":{"deposit":"f30c8d7a-37dc-47f4-a3d9-5586e9d31cb6"},"_deposit":{"created_by":3,"id":"14221","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"14221"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00014221","sets":["1132:1133:1134"]},"author_link":["93","903","62","108","24424","24425"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-01-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"10","bibliographicPageEnd":"1548","bibliographicPageStart":"1540","bibliographicVolumeNumber":"98","bibliographic_titles":[{"bibliographic_title":"Cancer Science"}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Chronic infection with hepatitis B virus (HBV) is a major risk factor for hepatocellular carcinoma. The HBV X protein (HBx) is thought to have oncogenic potential, although the molecular mechanism remains obscure. Pathological roles of HBx in the carcinogenic process have been examined using rodent systems and no report is available on the oncogenic roles of HBx in human cells in vitro. We therefore examined the effect of HBx on immortalization and transformation in human primary cells. We found that HBx could overcome active RAS-induced senescence in human immortalized cells and that these cells could form colonies in soft agar and tumors in nude mice. HBx alone, however, could contribute to neither immortalization nor transformation of these cells. In a population doubling analysis, an N-terminal truncated mutant of HBx, HBx-D1 (amino acids 51-154), which harbors the coactivation domain, could overcome active RAS-induced cellular senescence, but these cells failed to exhibit colonigenic and tumorigenic abilities, probably due to the low expression level of the protein. By scanning a HBx expression library of the clustered-alanine substitution mutants, the N-terminal domain was found to be critical for overcoming active RAS-induced senescence by stabilizing full-length HBx. These results strongly suggest that HBx can contribute to carcinogenesis by overcoming active oncogene-induced senescence. © 2007 Japanese Cancer Association.","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"医薬保健研究域医学系","subitem_description_type":"Other"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japanese Cancer Association / Blackwell Publishing Ltd"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1111/j.1349-7006.2007.00579.x","subitem_relation_type_select":"DOI"}}]},"item_4_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.jca.gr.jp/","subitem_relation_type_select":"URI"}}]},"item_4_rights_23":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© Japanese Cancer Association 日本癌学会"}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA11808050","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1347-9032","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Oishi, Naoki"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Shilagardi, Khurts"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakamoto, Yasunari"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Honda, Masao"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Kaneko, Shuichi"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Murakami, Seishi"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-03"}],"displaytype":"detail","filename":"ME-PR-KANEKO-S-1540.pdf","filesize":[{"value":"702.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ME-PR-KANEKO-S-1540.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/14221/files/ME-PR-KANEKO-S-1540.pdf"},"version_id":"e40313f5-72ed-45c9-b9ea-7d70f19e84ef"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Hepatitis B virus X protein overcomes oncogenic RAS-induced senescence in human immortalized cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Hepatitis B virus X protein overcomes oncogenic RAS-induced senescence in human immortalized cells"}]},"item_type_id":"4","owner":"3","path":["1134"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-03"},"publish_date":"2017-10-03","publish_status":"0","recid":"14221","relation_version_is_last":true,"title":["Hepatitis B virus X protein overcomes oncogenic RAS-induced senescence in human immortalized cells"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-07-28T00:50:56.526078+00:00"}