| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2017-10-03 |
| タイトル |
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|
タイトル |
Low molecular weight heparin suppresses receptor for advanced glycation end products-mediated expression of malignant phenotype in human fibrosarcoma cells |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| ID登録 |
|
|
ID登録 |
10.24517/00014342 |
|
ID登録タイプ |
JaLC |
| 著者 |
Takeuchi, Akihiko
Yamamoto, Yasuhiko
Munesue, Seiichi
Harashima, Ai
Watanabe, Takuo
Yonekura, Hideto
Yamamoto, Hiroshi
Tsuchiya, Hiroyuki
|
| 著者別表示 |
武内, 章彦
山本, 靖彦
棟居, 聖一
原島, 愛
渡邉, 琢夫
米倉, 秀人
山本, 博
土屋, 弘行
|
| 提供者所属 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
医薬保健研究域医学系 |
| 書誌情報 |
Cancer Science
巻 104,
号 6,
p. 740-749,
発行日 2013-06-01
|
| ISSN |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1347-9032 |
| NCID |
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|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11808050 |
| DOI |
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|
関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
10.1111/cas.12133 |
| 出版者 |
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出版者 |
Japanese Cancer Association / Blackwell Publishing Ltd |
| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
The receptor for advanced glycation end products (RAGE) is a pattern-recognition receptor and its engagement by ligands such as high mobility group box 1 (HMGB1) is implicated in tumor growth and metastasis. Low molecular weight heparin (LMWH) has an antagonistic effect on the RAGE axis and is also reported to exert an antitumor effect beyond the known activity of anticoagulation. However, the link between the anti-RAGE and antitumor activities of LMWH has not yet to be fully elucidated. In this study, we investigated whether LMWH could inhibit tumor cell proliferation, invasion, and metastasis by blocking the RAGE axis using in vitro and in vivo assay systems. Stably transformed HT1080 human fibrosarcoma cell lines were obtained, including human full-length RAGE-overexpressing (HT1080RAGE), RAGE dominant-negative, intracellular tail-deleted RAGE-overexpressing (HT1080dnRAGE), and mock-transfected control (HT1080mock) cells. Confocal microscopy showed the expression of HMGB1 and RAGE in HT1080 cells. The LMWH significantly inhibited HMGB1-induced NFκB activation through RAGE using an NFκB-dependent luciferase reporter assay and the HT1080 cell lines. Overexpression of RAGE significantly accelerated, but dnRAGE expression attenuated HT1080 cell proliferation and invasion in vitro, along with similar effects on local tumor mass growth and lung metastasis in vivo. Treatment with LMWH significantly inhibited the migration, invasion, tumor formation, and lung metastasis of HT1080RAGE cells, but not of HT1080mock or HT1080dnRAGE cells. In conclusion, this study revealed that RAGE exacerbated the malignant phenotype of human fibrosarcoma cells, and that this exacerbation could be ameliorated by LMWH. It is suggested that LMWH has therapeutic potential in patients with certain types of malignant tumors. © 2013 Japanese Cancer Association. |
| 権利 |
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|
権利情報 |
© Japanese Cancer Association 日本癌学会 |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 関連URI |
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|
識別子タイプ |
URI |
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関連識別子 |
http://www.jca.gr.jp/ |
ME-PR-TAKEUCHI-A-740.pdf (2.2 MB)
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