{"created":"2023-07-27T06:29:47.434125+00:00","id":14552,"links":{},"metadata":{"_buckets":{"deposit":"7079d4f0-d3f1-4a77-87c0-65c021fd02d1"},"_deposit":{"created_by":3,"id":"14552","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"14552"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00014552","sets":["1132:1133:1134"]},"author_link":["25641","25636","25638","25640","41","25637","84","25639","490"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-07-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"11","bibliographicPageEnd":"2204","bibliographicPageStart":"2190","bibliographicVolumeNumber":"30","bibliographic_titles":[{"bibliographic_title":"EMBO Journal"}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Transcription factors and epigenetic modulators are involved in the maintenance of self-renewal in embryonic stem (ES) cells. Here, we demonstrate the existence of a regulatory loop in ES cells between Sox2, an indispensable transcription factor for self-renewal, and embryonic ectoderm development (Eed), an epigenetic modulator regulating histone methylation. We found that Sox2 and Eed positively regulate each other's expression. Interestingly, Sox2 overexpression suppressed the induction of differentiation-associated genes in Eed-deficient ES cells without restoring histone methylation. This Sox2-mediated suppression was prevented by knockdown of the histone acetyltransferase (HAT), Tip60 or Elp3, and Sox2 stimulated expression of these HATs. Furthermore, forced expression of either HAT resulted in repression of differentiation-associated genes in Eed-deficient cells. These results suggest that Sox2 overcame the phenotype of Eed-deficient ES cells by promoting histone acetylation. We also found that knockout of Eed and knockdown of these HATs synergistically enhanced the upregulation of differentiation-associated genes in ES cells. Taken together, our results suggest that the Eed/Sox2 regulatory loop contributes to the maintenance of self-renewal in ES cells by controlling histone methylation and acetylation.","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医薬保健研究域医学系","subitem_description_type":"Other"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Nature Publishing Group"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1038/emboj.2011.126","subitem_relation_type_select":"DOI"}}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA10627582","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0261-4189","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ura, Hiroki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Murakami, Kazuhiro"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Akagi, Tadayuki"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Kinoshita, Keita"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamagichi, Shukuro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Masui, Shinji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Niwa, Hitoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Koide, Hiroshi"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Yokota, Takashi"}],"nameIdentifiers":[{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-03"}],"displaytype":"detail","filename":"ME-PR-KOIDE-H-2190.pdf","filesize":[{"value":"1.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ME-PR-KOIDE-H-2190.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/14552/files/ME-PR-KOIDE-H-2190.pdf"},"version_id":"9c72f0c0-148f-4d54-b325-27022be38391"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Eed/Sox2 regulatory loop controls ES cell self-renewal through histone methylation and acetylation","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Eed/Sox2 regulatory loop controls ES cell self-renewal through histone methylation and acetylation"}]},"item_type_id":"4","owner":"3","path":["1134"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-03"},"publish_date":"2017-10-03","publish_status":"0","recid":"14552","relation_version_is_last":true,"title":["Eed/Sox2 regulatory loop controls ES cell self-renewal through histone methylation and acetylation"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-07-28T00:45:25.357295+00:00"}