{"created":"2023-07-27T06:29:48.076946+00:00","id":14562,"links":{},"metadata":{"_buckets":{"deposit":"b5d7d413-4a39-43cc-a410-4a0f881f0ea7"},"_deposit":{"created_by":3,"id":"14562","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"14562"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00014562","sets":["1132:1133:1134"]},"author_link":["25682","25681","15392","25684","25683","25680"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-03-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"10","bibliographicPageEnd":"3934","bibliographicPageStart":"3930","bibliographicVolumeNumber":"106","bibliographic_titles":[{"bibliographic_title":"Proceedings of the National Academy of Sciences of the United States of America"}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"GPR109B (HM74) is a putative G protein-coupled receptor (GPCR) whose cognate ligands have yet to be characterized. GPR109B shows a high degree of sequence similarity to GPR109A, another GPCR that was identified as a high-affinity nicotinic acid (niacin) receptor. However, the affinity of nicotinic acid to GPR109B is very low. In this study, we found that certain aromatic D-amino acids, including D-phenylalanine, D-tryptophan, and the metabolite of the latter, D-kynurenine, decreased the activity of adenylate cyclase in cells transfected with GPR109B cDNA through activation of pertussis toxin (PTX)-sensitive G proteins. These D-amino acids also elicited a transient rise of intracellular Ca2+ level in cells expressing GPR109B in a PTX-sensitive manner. In contrast, these D-amino acids did not show any effects on cells expressing GPR109A. We found that the GPR109B mRNA is abundantly expressed in human neutrophils. D-phenylalanine and D-tryptophan induced a transient increase of intracellular Ca2+ level and a reduction of cAMP levels in human neutrophils. Furthermore, knockdown of GPR109B by RNA interference inhibited the D-amino acids-induced decrease of cellular cAMP levels in human neutrophils. These D-amino acids induced chemotactic activity of freshly prepared human neutrophils. We also found that D-phenylalanine and D-tryptophan induced chemotactic responses in Jurkat cells transfected with the GPR109B cDNA but not in mock-transfected Jurkat cells. These results suggest that these aromatic D-amino acids elicit a chemotactic response in human neutrophils via activation of GPR109B. © 2009 by The National Academy of Sciences of the USA.","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医薬保健研究域医学系","subitem_description_type":"Other"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"National Academy of Sciences"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1073/pnas.0811844106","subitem_relation_type_select":"DOI"}}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA10808769","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0027-8424","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Irukayama-Tomobe, Yoko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Tanaka, Hirokazu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yokomizo, Takehiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hashidate-Yoshida, Tomomi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yanagisawa, Masashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sakurai, Takeshi"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-03"}],"displaytype":"detail","filename":"ME-PR-SAKURAI-T-3930.pdf","filesize":[{"value":"1.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ME-PR-SAKURAI-T-3930.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/14562/files/ME-PR-SAKURAI-T-3930.pdf"},"version_id":"68ce7b37-dd2b-4dec-8c0f-3570c4a4e415"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Aromatic D-amino acids act as chemoattractant factors for human leukocytes through a G protein-coupled receptor, GPR109B","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Aromatic D-amino acids act as chemoattractant factors for human leukocytes through a G protein-coupled receptor, GPR109B"}]},"item_type_id":"4","owner":"3","path":["1134"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-03"},"publish_date":"2017-10-03","publish_status":"0","recid":"14562","relation_version_is_last":true,"title":["Aromatic D-amino acids act as chemoattractant factors for human leukocytes through a G protein-coupled receptor, GPR109B"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-07-28T00:45:09.548165+00:00"}