{"created":"2023-07-27T06:29:48.460008+00:00","id":14568,"links":{},"metadata":{"_buckets":{"deposit":"3df044eb-368b-421c-a133-4ab1ce71e5a3"},"_deposit":{"created_by":3,"id":"14568","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"14568"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00014568","sets":["1132:1133:1134"]},"author_link":["25702","25703","258","25705","25707","47","174","25708","21404","25706","55","25704"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-05-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1-3","bibliographicPageEnd":"131","bibliographicPageStart":"121","bibliographicVolumeNumber":"634","bibliographic_titles":[{"bibliographic_title":"European Journal of Pharmacology"}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Therapeutic angiogenesis is a promising strategy for treating ischemia. The lysophospholipid mediator sphingosine-1-phosphate (S1P) acts on vascular endothelial cells to stimulate migration and tube formation, and plays the critical role in developmental angiogenesis. We developed poly(lactic-co-glycolic-acid) (PLGA)-based S1P-containing microparticles (PLGA-S1P), which are biodegradable and continuously release S1P, and studied the effects of PLGA-S1P on neovascularization in murine ischemic hindlimbs. Intramuscular injections of PLGA-S1P stimulated blood flow in C57BL/6 mice dose-dependently, with repeated administrations at a 3-day interval, rather than a single bolus or 6-day interval, over 28. days conferring the optimal stimulating effect. In Balb/c mice that exhibit limb necrosis and dysfunction due to retarded blood flow recovery, injections of PLGA-S1P stimulated blood flow with alleviation of limb necrosis and dysfunction. PLGA-S1P alone did not induce edema in ischemic limbs, and rather blocked vascular endothelial growth factor-induced edema. PLGA-S1P not only increased the microvessel densities in ischemic muscle, but promoted coverage of vessels with smooth muscle cells and pericytes, thus stabilizing vessels. PLGA-S1P stimulated Akt and ERK with increased phosphorylation of endothelial nitric oxide synthase in ischemic muscle. The effects of the nitric oxide synthase inhibitor, Nω-nitro-l-arginine methylester, showed that PLGA-S1P-induced blood flow stimulation was partially dependent on nitric oxide. Injections of PLGA-S1P also increased the expression of angiogenic factors and the recruitment of CD45-, CD11b- and Gr-1-positive myeloid cells, which are implicated in post-ischemic angiogenesis, into ischemic muscle. These results indicate that PLGA-based, sustained local delivery of S1P is a potentially useful therapeutic modality for stimulating post-ischemic angiogenesis. © 2010 Elsevier B.V.","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医薬保健研究域医学系","subitem_description_type":"Other"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier BV"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.ejphar.2010.02.038","subitem_relation_type_select":"DOI"}}]},"item_4_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.elsevier.com/locate/issn/00142999","subitem_relation_type_select":"URI"}}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA00639687","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0014-2999","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Qia, Xun"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Okamoto, Yasuo"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Murakawa, Tomomi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Wang, Fei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Oyama, Osamu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ohkawa, Ryunosuke"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yoshioka, Kazuaki"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Du, Wa"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sugimoto, Naotoshi"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Yatomi, Yutaka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takuwa, Noriko"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Takuwa, Yoh"}],"nameIdentifiers":[{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-03"}],"displaytype":"detail","filename":"ME-PR-TAKUWA-Y-121.pdf","filesize":[{"value":"746.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ME-PR-TAKUWA-Y-121.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/14568/files/ME-PR-TAKUWA-Y-121.pdf"},"version_id":"e4a03edb-3957-46de-9b09-52af2b26ae04"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Sustained delivery of sphingosine-1-phosphate using poly(lactic-co-glycolic acid)-based microparticles stimulates Akt/ERK-eNOS mediated angiogenesis and vascular maturation restoring blood flow in ischemic limbs of mice","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Sustained delivery of sphingosine-1-phosphate using poly(lactic-co-glycolic acid)-based microparticles stimulates Akt/ERK-eNOS mediated angiogenesis and vascular maturation restoring blood flow in ischemic limbs of mice"}]},"item_type_id":"4","owner":"3","path":["1134"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-03"},"publish_date":"2017-10-03","publish_status":"0","recid":"14568","relation_version_is_last":true,"title":["Sustained delivery of sphingosine-1-phosphate using poly(lactic-co-glycolic acid)-based microparticles stimulates Akt/ERK-eNOS mediated angiogenesis and vascular maturation restoring blood flow in ischemic limbs of mice"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-07-28T00:45:03.667205+00:00"}