@article{oai:kanazawa-u.repo.nii.ac.jp:00014592,
 author = {Hitomi, Yoshiaki and Okuda, Junna and Nishino, Hirohito and Kambayashi, Yasuhiro and Hibino, Yuri and Takemoto, Kei and Takigawa, Tomoko and Ohno, Hideki and Taniguchi, Naoyuki and Ogino, Keiki},
 issue = {4},
 journal = {Journal of Biochemistry},
 month = {Apr},
 note = {3-Nitrotyrosine (NTyr) is considered as a biomarker of the generation of reactive nitrogen species (RNS). However, it is still difficult to determine its concentration in biological samples. To develop a reliable and high-throughput method, we optimized the conditions for high performance liquid chromatography and electrochemical detection (HPLC-ECD). The best separation of NTyr was achieved using a highly acidic mobile phase (pH 2.5). The concentration of protein-bound NTyr in plasma protein was 593.6 ± 53.8 fmol/mg in rats treated with lipopolysaccharide (LPS) and 114.4 ± 27.6 fmol/mg in control. After intravenous administration of in vitro-nitrated plasma protein, NTyr concentration decreased; the half-life was 63.4 ± 16.8 h. Consistently, protein-bound NTyr concentration in plasma after LPS treatment declined gradually, but was detectable for 1 week. Our protocol is reproducible and suitable for analysing multiple clinical samples to study RNS production in vivo. © 2007 The Japanese Biochemical Society., 金沢大学医薬保健研究域医学系},
 pages = {495--502},
 title = {Disposition of protein-bound 3-nitrotyrosine in rat plasma analysed by a novel protocol for HPLC-ECD},
 volume = {141},
 year = {2007}
}