{"created":"2023-07-27T06:29:49.664440+00:00","id":14592,"links":{},"metadata":{"_buckets":{"deposit":"c00f8771-7bdd-4d20-9e9a-4b7a82fe4db8"},"_deposit":{"created_by":3,"id":"14592","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"14592"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00014592","sets":["1132:1133:1134"]},"author_link":["25817","25821","25818","25822","21925","170","88","21891","25819","25820"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-04-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"502","bibliographicPageStart":"495","bibliographicVolumeNumber":"141","bibliographic_titles":[{"bibliographic_title":"Journal of Biochemistry"}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"3-Nitrotyrosine (NTyr) is considered as a biomarker of the generation of reactive nitrogen species (RNS). However, it is still difficult to determine its concentration in biological samples. To develop a reliable and high-throughput method, we optimized the conditions for high performance liquid chromatography and electrochemical detection (HPLC-ECD). The best separation of NTyr was achieved using a highly acidic mobile phase (pH 2.5). The concentration of protein-bound NTyr in plasma protein was 593.6 ± 53.8 fmol/mg in rats treated with lipopolysaccharide (LPS) and 114.4 ± 27.6 fmol/mg in control. After intravenous administration of in vitro-nitrated plasma protein, NTyr concentration decreased; the half-life was 63.4 ± 16.8 h. Consistently, protein-bound NTyr concentration in plasma after LPS treatment declined gradually, but was detectable for 1 week. Our protocol is reproducible and suitable for analysing multiple clinical samples to study RNS production in vivo. © 2007 The Japanese Biochemical Society.","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医薬保健研究域医学系","subitem_description_type":"Other"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本生化学会 = Japanese Biochemical Society"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1093/jb/mvm050","subitem_relation_type_select":"DOI"}}]},"item_4_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1093/jb/mvm050","subitem_relation_type_select":"DOI"}},{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://jb.oxfordjournals.org/cgi/content/abstract/141/4/495","subitem_relation_type_select":"URI"}}]},"item_4_rights_23":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2008 The Japanese Biochemical Society."}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA00694073","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0021-924X","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hitomi, Yoshiaki"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Okuda, Junna"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nishino, Hirohito"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kambayashi, Yasuhiro"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Hibino, Yuri"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Takemoto, Kei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takigawa, Tomoko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ohno, Hideki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Taniguchi, Naoyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ogino, Keiki"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-03"}],"displaytype":"detail","filename":"ME-PR-HITOMI-Y-495.pdf","filesize":[{"value":"310.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ME-PR-HITOMI-Y-495.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/14592/files/ME-PR-HITOMI-Y-495.pdf"},"version_id":"aceb8cae-80c8-460c-828b-14eabab47e8e"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Disposition of protein-bound 3-nitrotyrosine in rat plasma analysed by a novel protocol for HPLC-ECD","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Disposition of protein-bound 3-nitrotyrosine in rat plasma analysed by a novel protocol for HPLC-ECD"}]},"item_type_id":"4","owner":"3","path":["1134"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-03"},"publish_date":"2017-10-03","publish_status":"0","recid":"14592","relation_version_is_last":true,"title":["Disposition of protein-bound 3-nitrotyrosine in rat plasma analysed by a novel protocol for HPLC-ECD"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-07-28T00:44:32.975349+00:00"}