{"created":"2023-07-27T06:29:53.762542+00:00","id":14689,"links":{},"metadata":{"_buckets":{"deposit":"c2763d02-f058-442b-aa6a-36be08848e29"},"_deposit":{"created_by":3,"id":"14689","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"14689"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00014689","sets":["1132:1133:1134"]},"author_link":["26120","26121","26123","26122","88","21925","170","21891","44"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-01-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"103","bibliographicPageStart":"95","bibliographicVolumeNumber":"44","bibliographic_titles":[{"bibliographic_title":"Journal of Clinical Biochemistry and Nutrition"}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"(Di)bromotyrosine is formed by the specific reaction of eosinophil peroxidase and can be used as an eosinophil activation marker. In the present study, an antibody for (di)bromotyrosine in proteins was prepared to investigate the pathogenesis of eosinophil-related diseases such as allergic responses. A rabbit polyclonal antibody was raised against brominated keyhole limpet hemocyanin. The specificity of the antiserum was investigated with an enzyme-linked immunosorbent assay (ELISA). The antiserum recognized brominated bovine serum albumin (BSA) and dibromotyrosine-conjugated BSA. The antiserum also reacted with chlorinated BSA and di-iodotyrosine-conjugated BSA. Moreover, the specificity of the antiserum was investigated using competitive ELISA. Dibromotyrosine and di-iodotyrosine inhibited the recognition of brominated BSA by the antiserum. However, the recognition of brominated BSA by the antiserum was not inhibited by bromotyrosine, chlorotyrosine, iodotyrosine, nitrotyrosine, aminotyrosine, phosphotyrosine, or tyrosine. These results suggested that the epitope of the antiserum is dihalogenated tyrosine. Immunohistochemically, the antiserum stained brominated rat eosinophils but not chlorinated or nitrated eosinophils. In conclusion, an antiserum for dihalogenated protein was prepared. It is expected that the antiserum will be useful for the analysis of the pathogenesis of allergic diseases such as asthma and atopic dermatitis.","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医薬保健研究域医学系","subitem_description_type":"Other"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本酸化ストレス学会 = SFRR Japan"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3164/jcbn.08-196","subitem_relation_type_select":"DOI"}}]},"item_4_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.jstage.jst.go.jp/article/jcbn/44/1/44_95/_article","subitem_relation_type_select":"URI"}}]},"item_4_rights_23":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright (c) 2008 by The Editorial Secretariat of JCBN"}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA10710201","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0912-0009","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kambayashi, Yasuhiro"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Ogino, Keiki"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Takemoto, Kei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Imagama, Takashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Takigawa, Tomoko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kimura, Shingo"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hibino, Yuri"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Hitomi, Yoshiaki"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Nakamura, Hiroyuki"}],"nameIdentifiers":[{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-03"}],"displaytype":"detail","filename":"ME-PR-KAMBAAYASHI-Y-95.pdf","filesize":[{"value":"429.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"ME-PR-KAMBAAYASHI-Y-95.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/14689/files/ME-PR-KAMBAAYASHI-Y-95.pdf"},"version_id":"e46c360e-987f-4939-b790-b25d6150a234"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Efficient assay for total antioxidant capacity in human plasma using a 96-well microplte","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Efficient assay for total antioxidant capacity in human plasma using a 96-well microplte"}]},"item_type_id":"4","owner":"3","path":["1134"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-03"},"publish_date":"2017-10-03","publish_status":"0","recid":"14689","relation_version_is_last":true,"title":["Efficient assay for total antioxidant capacity in human plasma using a 96-well microplte"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-07-28T00:42:37.111079+00:00"}