@article{oai:kanazawa-u.repo.nii.ac.jp:00014778,
 author = {Kakimoto, Kensaku and Toriba, Akira and Ohno, Takanori and Ueno, Mariko and Kameda, Takayuki and Tang, Ning and Hayakawa, Kazuichi},
 issue = {2},
 journal = {Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences},
 month = {May},
 note = {To evaluate human exposure to polycyclic aromatic hydrocarbons (PAHs), we developed a rapid, simple and sensitive method for determining 1-hydroxypyrene-glucuronide (1-OHP-G) in human urine. To improve precision, a deuterated glucuronide was used as an internal standard. The method requires only 1 mL of urine. The urine was treated with a mixed-mode anion-exchange and reversed-phase solid-phase extraction cartridge (Oasis MAX). The analytes were analyzed with a C18 reversed-phase column with a gradient elution, followed by tandem mass spectrometry with electrospray ionization in negative ion mode. The detection limit of 1-OHP-G (corresponding to a signal-to-noise ratio of 3) was 0.13 fmol/injection. Urinary concentrations of 1-OHP-G determined by this method were strongly correlated (r2 = 0.961) with concentrations of 1-hydroxypyrene by conventional HPLC with fluorescence detection. © 2008 Elsevier B.V. All rights reserved., 金沢大学医薬保健研究域薬学系},
 pages = {259--263},
 title = {Direct measurement of the glucuronide conjugate of 1-hydroxypyrene in human urine by using liquid chromatography with tandem mass spectrometry},
 volume = {867},
 year = {2008}
}