@article{oai:kanazawa-u.repo.nii.ac.jp:00014841,
 author = {Takano, Fumihide and Yamaguchi, Masaya and Takada, Satoko and Shoda, Satoko and Yahagi, Nobuo and Takahashi, Tomoya and Ohta, Tomihisa},
 issue = {17},
 journal = {Life Sciences},
 month = {Apr},
 note = {We investigated the effects of red pepper (Capsicum annuum Lin.) extracts (capsicum extract) and its main pungent capsaicin on T helper 1 (Th1) and 2 (Th2) cytokine production in cultured murine Peyer's patch (PP) cells in vitro and ex vivo. Direct administration of capsicum extract (1 and 10 μg/ml) and capsaicin (3 and 30 μM) resulted in suppression of interleukin (IL)-2, interferon (IFN)-γ, IL-4 and IL-5 production. In an ex vivo experiment using PP cells removed from the mice after oral administration of capsicum extract (10 mg/kg/day for 4 consecutive days), IL-2, IFN-γ and IL-5 increased in response to concanavalin A (Con A). Oral administration of 3 mg/kg/day capsaicin, one active constituent of the extract, also enhanced IL-2, INF-γ and IL-4 production in response to Con A stimulation but did not influence the production of IL-5. Orally administered capsazepine (3 mg/kg/day), a selective transient receptor potential vanilloid 1 (TRPV1) antagonist, slightly enhanced IL-2 production also irrespective of Con A stimulation. The capsaicin-induced enhancement of both IL-2 and IFN-γ production was not reduced by oral administration of capsazepine (3 mg/kg/day), suggesting a TRPV1 receptor-independent mechanism. Flow cytometric analysis revealed that the population of CD3+ cells in the PP cells was significantly reduced while CD19+ cells increased after oral administration of capsicum extract (1 and 10 mg/kg/day) and capsaicin (0.3 and 3 mg/kg/day). Capsazepine (3 mg/kg/day) weakly but significantly reversed these effects. Orally administered capsicum extract and capsaicin did not change the T cell subset (CD4+ and CD8+), Th1 (IFN-γ+) and T2 (IL-4+) ratio. These findings indicate that capsicum extract and capsaicin modulate T cell-immune responses, and their immunomodulatory effects on murine PP cells are partly due to both TRPV1-dependent and -independent pathway. © 2007 Elsevier Inc. All rights reserved., 金沢大学大学院自然科学研究科生理活性物質科学, 金沢大学薬学部},
 pages = {1553--1563},
 title = {Capsicum ethanol extracts and capsaicin enhance interleukin-2 and interferon-gamma production in cultured murine Peyer's patch cells ex vivo},
 volume = {80},
 year = {2007}
}