{"created":"2023-07-27T06:30:01.891887+00:00","id":14881,"links":{},"metadata":{"_buckets":{"deposit":"c23701cb-d7d2-4ef8-989b-1eaefbf5c2a3"},"_deposit":{"created_by":3,"id":"14881","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"14881"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00014881","sets":["1132:1133:1135"]},"author_link":["227","185","26698","26700","26701","26699"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-02-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"447","bibliographicPageStart":"440","bibliographicVolumeNumber":"29","bibliographic_titles":[{"bibliographic_title":"Carcinogenesis"}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Accumulating evidence suggests that platelet-type 12-lipoxygenase (p12-LOX) plays an important role in tumor development. However, how p12-LOX contributes to tumorigenesis is still not understood. The role of p12-LOX was therefore examined in tumor promotion using mouse epidermal JB6 P+ cells that are sensitive to 12-O- tetradecanoylphorbol-13-acetate-induced transformation. The expression of p12-LOX was significantly higher in JB6 P+ cells than in JB6 P- cells that were resistant to transformation, and its expression was further increased by tumor necrosis factor (TNF)-α. Importantly, the inhibition of p 12-LOX in JB6 P+ cells by baicalein, a specific inhibitor or small interfering RNA significantly suppressed TPA-induced transformation. Moreover, treatment with 12(S)-hydroxyeicosatetraenoic acid (HETE), a metabolite of p12-LOX, enhanced TPA-induced neoplastic transformation either in the presence or absence of baicalein. These results indicate that p12-LOX is required for tumor promotion of epidermal cells and that 12(S)-HETE functions as a rate-limiting factor. Notably, treatment with baicalein significantly suppressed the proliferation of JB6 P+ cells when cells were seeded at a low density in a culture plate. Moreover, the cloning efficiency of JB6 P+ cells was dramatically decreased by inhibition of p12-LOX. In contrast, baicalein treatment did not affect the cloning efficiency of most malignant cancer cells. These results indicate that p12-LOX is induced by the inflammatory cytokine TNF-α in the early stage of tumorigenesis, and is required for tumor promotion through enhancing efficient proliferation of a small number of initiated cells. The present results suggest that the p12-LOX pathway may be an effective target of chemoprevention for skin carcinogenesis. © The Author 2008. Published by Oxford University Press. All rights reserved.","subitem_description_type":"Abstract"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Oxford University Press"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1093/carcin/bgm274","subitem_relation_type_select":"DOI"}}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA00141799","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0143-3334","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Du, Yu-Chen"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Oshima, Hiroko"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Jin, Jing-Chun"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nomura, Masaaki"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Yoshimoto, Tanihiro"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Oshima, Masanobu"}],"nameIdentifiers":[{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-03"}],"displaytype":"detail","filename":"PH-PR-PIAO-Y-S-440.pdf","filesize":[{"value":"182.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"PH-PR-PIAO-Y-S-440.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/14881/files/PH-PR-PIAO-Y-S-440.pdf"},"version_id":"044f515c-baa6-4239-a9e1-7985c7b256b4"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Platelet-type 12-lipoxygenase accelerates tumor promotion of mouse epidermal cells through enhancement of cloning efficiency","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Platelet-type 12-lipoxygenase accelerates tumor promotion of mouse epidermal cells through enhancement of cloning efficiency"}]},"item_type_id":"4","owner":"3","path":["1135"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-03"},"publish_date":"2017-10-03","publish_status":"0","recid":"14881","relation_version_is_last":true,"title":["Platelet-type 12-lipoxygenase accelerates tumor promotion of mouse epidermal cells through enhancement of cloning efficiency"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-07-28T00:40:39.073595+00:00"}