{"created":"2023-07-27T06:30:02.243415+00:00","id":14889,"links":{},"metadata":{"_buckets":{"deposit":"6967fcec-df69-4344-93c2-bd777b852870"},"_deposit":{"created_by":3,"id":"14889","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"14889"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00014889","sets":["4161:3020:3021"]},"author_link":["26717","26718","26720","26715","196","26719","76362","26714","2309","95962","26716","26721","63"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-01-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"11","bibliographicPageEnd":"2526","bibliographicPageStart":"2520","bibliographicVolumeNumber":"119","bibliographic_titles":[{"bibliographic_title":"International Journal of Cancer"}]}]},"item_4_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"中嶋, 美紀 "}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"深見, 達基"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"山崎, 浩史"}],"nameIdentifiers":[{},{}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Cigarette smoking is the predominant risk factor for bladder cancer. Aromatic amines such as 4-aminobiphenyl (ABP) is the major carcinogens found in tobacco smoke. Although it is generally accepted that ABP is metabolically activated via N-hydroxylation by CYP1A2 in human liver, previous studies using Cyp1a2-null mice indicated the involvement of other enzyme(s). Here we found that CYP2A13 can metabolically activate ABP to show genotoxicity by Umu assay. The Km and Vmax values for ABP N-hydroxylation by recombinant CYP2A13 in E. coli were 38.5 ± 0.6 μM 7.8 ± 0.0 pmol/min/pmol CYP, respectively. The Km and Vmax values by recombinant CYP1A2 were 9.9 ± 0.9 μM and 39.6 ± 0.9 pmol/min/ pmol CYP, respectively, showing 20-fold higher intrinsic clearance than CYP2A13. In human bladder, CYP2A13 mRNA, but not CYP1A2, is expressed at a relatively high level. Human bladder microsomes showed ABP N-hydroxylase activity (K m = 34.9 ± 4.7 μM and Vmax = 57.5 ± 1.9 pmol/min/mg protein), although the intrinsic clearance was 5-fold lower than that in human liver microsomes (Km = 33.2 ± 2.0 μM and Vmax = 293.9 ± 5.8 pmol/min/mg protein). The activity in human bladder microsomes was prominently inhibited by 8-methoxypsoralen, but not by fluvoxamine, anti-CYP1A2 or anti-CYP2A6 antibodies.","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学ナノ生命科学研究所 / 金沢大学大学院医学系研究科機能分子医薬学 / 金沢大学薬学部","subitem_description_type":"Other"}]},"item_4_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00014876","subitem_identifier_reg_type":"JaLC"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Wiley-Liss"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1002/ijc.22136","subitem_relation_type_select":"DOI"}}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0020-7136","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nakajima, Miki"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Itoh, Masahiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Sakai, Haruko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fukami, Tatsuki"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Kato, Miki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamazaki, Hiroshi"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Kadlubar, Fred F."}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Imaoka, Susumu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Funae, Yoshihiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yokoi, Tsuyoshi"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-03"}],"displaytype":"detail","filename":"PH-PR-NAKAJIMA-M-2520.pdf","filesize":[{"value":"554.0 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"PH-PR-NAKAJIMA-M-2520.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/14889/files/PH-PR-NAKAJIMA-M-2520.pdf"},"version_id":"df426ae8-78e4-4789-ae62-f20aeddf8ea1"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"CYP2A13 expressed in human bladder metabolically activates 4-aminobiphenyl","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"CYP2A13 expressed in human bladder metabolically activates 4-aminobiphenyl"}]},"item_type_id":"4","owner":"3","path":["3021"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-03"},"publish_date":"2017-10-03","publish_status":"0","recid":"14889","relation_version_is_last":true,"title":["CYP2A13 expressed in human bladder metabolically activates 4-aminobiphenyl"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-07-27T10:12:23.372323+00:00"}