@article{oai:kanazawa-u.repo.nii.ac.jp:00014991,
 author = {Hosozumi, Chiemi and Toriba, Akira and Chuesaard, Thanyarat and Kameda, Takayuki and Tang, Ning and Hayakawa, Kazuichi},
 journal = {Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences},
 month = {Apr},
 note = {Urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) is a widely used noninvasive biomarker of oxidative stress. A selective, sensitive and rapid method for determining 8-OHdG in human urine was developed using hydrophilic interaction chromatography-tandem mass spectrometry (HILIC-MS/MS) with electrospray ionization. 8-OHdG and isotopically labeled 8-OHdG (internal standard) were separated on a HILIC column with a mobile phase of 10. mM ammonium acetate: acetonitrile (1:9, v/v) within 10. min and detected by using a positive electrospray ionization interface under the selected reaction monitoring mode. The detection limits of 8-OHdG (corresponding to a signal-to-noise ratio of 3) for the HILIC-MS/MS system and the conventional method using a reversed-phase column with MS/MS were 1.0 and 26.0. fmol/injection, respectively. The proposed method makes it possible to monitor the basal level of urinary 8-OHdG from non-exposed healthy subjects and can be used for large-scale human studies. © 2012 Elsevier B.V.},
 pages = {173--176},
 title = {Analysis of 8-hydroxy-2'-deoxyguanosine in human urine using hydrophilic interaction chromatography with tandem mass spectrometry},
 volume = {893-894},
 year = {2012}
}