{"created":"2023-07-27T06:30:12.224111+00:00","id":15122,"links":{},"metadata":{"_buckets":{"deposit":"e7e081c7-8ca9-4269-8380-870c7e9bdf78"},"_deposit":{"created_by":3,"id":"15122","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"15122"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00015122","sets":["4161:3020:3021"]},"author_link":["27286","76362","196","2309","26716","27288","27287","63"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-03-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"794","bibliographicPageStart":"780","bibliographicVolumeNumber":"31","bibliographic_titles":[{"bibliographic_title":"Pharmaceutical Research"}]}]},"item_4_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"深見, 達基"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"横井, 毅"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"中嶋, 美紀 "}],"nameIdentifiers":[{},{},{}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Purpose: Cytochrome b 5 (b 5) is a hemoprotein that transfers electrons to several enzymes to fulfill functions in fatty acid desaturation, methemoglobin reduction, steroidogenesis, and drug metabolism. Despite the importance of b 5, the regulation of b 5 expression in human liver remains largely unknown. We investigated whether microRNA (miRNA) might be involved in the regulation of human b 5. Methods: Twenty-four human liver specimens were used for correlation analysis. In silico analysis and luciferase assay were performed to determine whether the predicted miRNAs functionally target to b 5. The miR-223 was overexpressed into HepG2 cells infected with adenovirus expressing human cytochrome P450. Results: In human livers, the b 5 protein levels were not positively correlated with the b 5 mRNA levels, and miR-223 levels were inversely correlated with the b 5 mRNA levels or the translational efficiencies. The luciferase assay showed that miR-223 functionally binds to the element in the 3′-untranslated region of b 5 mRNA. The overexpression of miR-223 significantly reduced the endogenous b 5 protein level and the mRNA stability in HepG2 cells. Moreover, the overexpression of miR-223 significantly reduced CYP3A4-catalyzed testosterone 6β-hydroxylation activity and CYP2E1-catalyzed chlorzoxazone 6-hydroxylase activity but not CYP1A2-catalyzed 7-ethoxyresorufin O-deethylase activity. Conclusions: miR-223 down-regulates b 5 expression in the human liver, modulating P450 activities. © 2013 Springer Science+Business Media New York.","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学ナノ生命科学研究所 / 金沢大学医薬保健研究域薬学系","subitem_description_type":"Other"}]},"item_4_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00015109","subitem_identifier_reg_type":"JaLC"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1007/s11095-013-1200-7","subitem_relation_type_select":"DOI"}}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA10632083","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0724-8741","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Takahashi, Kei"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Oda, Yuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Toyoda, Yasuyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Fukami, Tatsuki"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Yokoi, Tsuyoshi"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Nakajima, Miki"}],"nameIdentifiers":[{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-04"}],"displaytype":"detail","filename":"PH-PR-NAKAJIMA-M-780.pdf","filesize":[{"value":"617.5 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"PH-PR-NAKAJIMA-M-780.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/15122/files/PH-PR-NAKAJIMA-M-780.pdf"},"version_id":"d3135957-7859-4a93-8616-fed91a1d35e8"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Regulation of Cytochrome b5 Expression by miR-223 in Human Liver: Effects on Cytochrome P450 Activities","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Regulation of Cytochrome b5 Expression by miR-223 in Human Liver: Effects on Cytochrome P450 Activities"}]},"item_type_id":"4","owner":"3","path":["3021"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-04"},"publish_date":"2017-10-04","publish_status":"0","recid":"15122","relation_version_is_last":true,"title":["Regulation of Cytochrome b5 Expression by miR-223 in Human Liver: Effects on Cytochrome P450 Activities"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-07-27T10:11:44.179005+00:00"}