@article{oai:kanazawa-u.repo.nii.ac.jp:00015185, author = {Hayakawa, Kazuichi and Nakamura, Seiji and Inaki, Kotoe and Miyazaki, Motoichi}, issue = {6}, journal = {Analytical Sciences}, month = {Jan}, note = {Carboxylesters, carboxylic and inorganic anions were determined separately by photometric ion chromatography with an enzyme immobilized column. In the system, a carboxylesterase immobilized column (4.6mm i.d.×10mm), which converted carboxylesters to carboxylic anions completely, was set between a sample injector and an analytical column. The other chromatographic conditions were as follows: an anion exchanger-type separation column (4.6mm i.d.×250mm); phthalate-N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid e uent (pH 6.5); an oven of temperature 40°C; a flow rate of 1.0ml/min; 280-nm UV detection; and an injection volume of 20μl. Calibration curves for ethyl esters of acetate, propionate, butyrate and isova erate were linear with the range 5×10-10mol to 1×10-7mol according to the peak-height method; their detection limits were 10-10 mol levels. The present method was applied for the determination of carboxylesters in practical samples.}, pages = {691--695}, title = {Determination of Carboxylesters by Indirect Photometric Ion Chromatography with an On-Line Enzyme Immobilized Column}, volume = {5}, year = {1989} }