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Studies on Neurosteroids XXI: An Improved Liquid Chromatography-Tandem Mass Spectrometric Method for Determination of 5α-Androstane-3α,17β-diol in Rat Brains
http://hdl.handle.net/2297/28460
http://hdl.handle.net/2297/28460edc21380-a8d1-4875-b44e-0fe445ddf21b
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
PH-PR-SHIMADA-K-1015.pdf (92.7 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2017-10-04 | |||||
| タイトル | ||||||
| タイトル | Studies on Neurosteroids XXI: An Improved Liquid Chromatography-Tandem Mass Spectrometric Method for Determination of 5α-Androstane-3α,17β-diol in Rat Brains | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Higashi, Tatsuya
× Higashi, Tatsuya× Nishino, Tadashi× Yokoi, Hiroyuki× Ninomiya, Yasuaki× Shimada, Kazutake |
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| 書誌情報 |
Analytical sciences 巻 23, 号 8, p. 1015-1019, 発行日 2007-08-10 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0910-6340 | |||||
| NCID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA10500785 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.2116/analsci.23.1015 | |||||
| 出版者 | ||||||
| 出版者 | 日本分析化学会 = The Japan Society for Analytical Chemistry | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | A sensitive liquid chromatography-electrospray ionization-tandem mass spectrometric (LC-ESI-MS/MS) method for the determination of the rat brain 5α-androstane-3α,17β-diol (3α,5α-Adiol) has been developed and validated. The brain extract was purified using solid-phase extraction cartridges, derivatized with isonicotinoyl azide, and subjected to LC-MS/MS. The method was accurate and reproducible, and the limit of quantitation was 0.1 ng/g tissue when a 100-mg tissue sample was used. The change in the brain 3α,5α-Adiol level by immobilization stress was also analyzed using the developed method. | |||||
| 権利 | ||||||
| 権利情報 | Copyright (c) 2007 by The Japan Society for Analytical Chemistry | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 関連URI | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://wwwsoc.nii.ac.jp/jsac/ | |||||
| 関連URI | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://ci.nii.ac.jp/naid/10019747846 | |||||
| 関連URI | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://japanlinkcenter.org/JST.JSTAGE/analsci/23.1015 | |||||
