@article{oai:kanazawa-u.repo.nii.ac.jp:00015242,
 author = {Higashi, Tatsuya and Nishino, Tadashi and Yokoi, Hiroyuki and Ninomiya, Yasuaki and Shimada, Kazutake},
 issue = {8},
 journal = {Analytical sciences},
 month = {Aug},
 note = {A sensitive liquid chromatography-electrospray ionization-tandem mass spectrometric (LC-ESI-MS/MS) method for the determination of the rat brain 5α-androstane-3α,17β-diol (3α,5α-Adiol) has been developed and validated. The brain extract was purified using solid-phase extraction cartridges, derivatized with isonicotinoyl azide, and subjected to LC-MS/MS. The method was accurate and reproducible, and the limit of quantitation was 0.1 ng/g tissue when a 100-mg tissue sample was used. The change in the brain 3α,5α-Adiol level by immobilization stress was also analyzed using the developed method.},
 pages = {1015--1019},
 title = {Studies on Neurosteroids XXI: An Improved Liquid Chromatography-Tandem Mass Spectrometric Method for Determination of 5α-Androstane-3α,17β-diol in Rat Brains},
 volume = {23},
 year = {2007}
}