{"created":"2023-07-27T06:30:19.889419+00:00","id":15309,"links":{},"metadata":{"_buckets":{"deposit":"4a7b5759-7590-45b9-9de7-03fcf200b959"},"_deposit":{"created_by":3,"id":"15309","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"15309"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00015309","sets":["1132:1133:1135"]},"author_link":["20795","27738","27739","27740","53","27737","313"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-04-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"769","bibliographicPageStart":"761","bibliographicVolumeNumber":"139","bibliographic_titles":[{"bibliographic_title":"Journal of Biochemistry"}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The phospho-site adapter protein 14-3-3 binds to target proteins at amino acid sequences matching the consensus motif Arg-X-X-Ser/Thr-X-Pro, where the serine or threonine residue is phosphorylated and X is any amino acid. The dual-specificity phosphatase CDC25B, which is involved in cell cycle regulation, contains five 14-3-3 binding motifs, but 14-3-3 preferentially binds to the motif at Ser309 in CDC25B1 (or Ser323 in CDC25B3). In the present study, we demonstrate that amino acid residues C-terminal to the 14-3-3 binding motif strongly affect the efficiency of 14-3-3 binding. Alanine substitutions at residues downstream of the Ser309 motif dramatically reduced 14-3-3 binding, although phosphorylation of Ser309 was unaffected. We also observed that binding of endogenous 14-3-3 to mutant CDC25B occurred less efficiently than to the wild type. Mutants to which 14-3-3 cannot bind efficiently tend to be located in the nucleus, although not as specifically as the alanine substitution mutant of Ser309. These results indicate that amino acid sequences C-terminal to the consensus binding site have an important role in the efficient binding of 14-3-3 to at least CDC25B, which may partly explain why some consensus sequences are inactive as 14-3-3 binding sites. © 2006 The Japanese Biochemical Society.","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学医薬保健研究域薬学系","subitem_description_type":"Other"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本生化学会 = Japanese Biochemical Society"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1093/jb/mvj079","subitem_relation_type_select":"DOI"}}]},"item_4_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://dx.doi.org/10.1093/jb/mvj079","subitem_relation_type_select":"DOI"}}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA00694073","subitem_source_identifier_type":"NCID"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Uchida, Sanae"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"Kubo, Akitsugu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kizu, Ryoichi"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Nakagama, Hitoshi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Matsunaga, Tsukasa"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Ishizaka, Yukihito"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamashita, Katsumi"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-04"}],"displaytype":"detail","filename":"PH-PR-YAMASHITA-K-761.pdf","filesize":[{"value":"354.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"PH-PR-YAMASHITA-K-761.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/15309/files/PH-PR-YAMASHITA-K-761.pdf"},"version_id":"11708b8f-b621-4f70-8762-3278c7166de3"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Amino acids C-terminal to the 14-3-3 binding motif in CDC25B affect the efficiency of 14-3-3 binding","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Amino acids C-terminal to the 14-3-3 binding motif in CDC25B affect the efficiency of 14-3-3 binding"}]},"item_type_id":"4","owner":"3","path":["1135"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-04"},"publish_date":"2017-10-04","publish_status":"0","recid":"15309","relation_version_is_last":true,"title":["Amino acids C-terminal to the 14-3-3 binding motif in CDC25B affect the efficiency of 14-3-3 binding"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-07-28T00:34:34.078141+00:00"}