{"created":"2023-07-27T06:30:21.738264+00:00","id":15353,"links":{},"metadata":{"_buckets":{"deposit":"8c9dd79b-9d05-4201-9a7d-f69bd63d14c9"},"_deposit":{"created_by":3,"id":"15353","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"15353"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00015353","sets":["1132:1133:1135"]},"author_link":["27856","27859","27858","27860","458","27857"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-03-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"198","bibliographicPageStart":"191","bibliographicVolumeNumber":"36","bibliographic_titles":[{"bibliographic_title":"薬理と治療 = Basic pharmacology and therapeutics"}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"本研究では, 田七人参(田七)の抗炎症作用の機序および活性成分の解明を目的に, 炎症と関係の深い8種類のサイトカイン(INF-γ, TNF-α, IL-1β, IL-2, IL-4, IL-5, IL-6およびIL-10)の分泌に対する作用を検討した. 田七のメタノール抽出エキス(田七エキス(A)：100μg/mL)は, それぞれIL-1β分泌を88％, TNF-α分泌を51％, IL-4分泌を34％, INF-γ分泌を28％, IL-10分泌を27％, IL-6分泌を13％抑制した. 他方, IL-2分泌には有意な影響を認めず, IL-5分泌を48％促進した. そこで分泌阻害効果がもっとも強かったIL-1β分泌についてさらに検討したところ, 田七エキス(A)の阻害率は, 30μg/mLで17％, および100μg/mLで78％であった. 一方, シリカゲル薄層クロマトグラフィー(展開溶媒としてCHCl3：MeOH：H2O＝7：3：0.5混合液, 展開距離15cm)でginsenoside Rg1およびそれよりRf値の低い画分(B)(人参と共通するginsenoside類を多く含む画分)のIL-1β分泌阻害率は, 200μg/mLの高濃度でも20％であり, きわめて低いことがわかった. しかし, Rf値の高い画分(C)はそのIC50が2.86μ/mLと, 有意なIL-1β分泌阻害作用を示すことが認められた. 本結果は, 田七の抗炎症作用が主としてIL-1βの分泌阻害作用を介して発現すること, その活性成分は人参に含まれない脂溶性の高いginsenoside類に起因することが強く示唆された. The aim of this research was to find the mechanism of anti-inflammatory effect and the properties of the active constituents of methanol extract of Panax notoginseng (Denchi) in vitro. Denchi (100 μg/mL) inhibited the release of IL-1/8 (88%), INF-α (51%), IL-4 (34%), INF-γ (28%), IL-10 (27%), IL-6 (13%) from PMBC. However, it did not change the IL-2 release, and increased the IL-5 release (48%). The fraction-C, in which denchi-specific ginsenosides were contained, showed strong and dose-related inhibition of IL-1β release (IC50 = 2.86 μg/mL). However, the inhibitory activity of Panax ginseng-related ginsenosides (fraction-B) was extremely weak, 20% at 200 μg/mL, as compared with that of the fraction-C. These results suggest that the anti-inflammatory effect of Denchi is achieved through an inhibition of the IL-1β release, and that the active compounds are highly lipid-soluble ginsenosides.","subitem_description_type":"Abstract"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"ライフサイエンス出版"}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AN00242029","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0386-3603","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"嶋田, 努"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"丸山, 博文"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"栗原, 久"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"丸山, 悠司"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"赤瀬, 智子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"油田, 正樹"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-04"}],"displaytype":"detail","filename":"PH-PR-SHIMADA-T-191.pdf","filesize":[{"value":"6.5 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"PH-PR-SHIMADA-T-191.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/15353/files/PH-PR-SHIMADA-T-191.pdf"},"version_id":"f5ca4527-412c-4a86-9da4-8410b222411b"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"田七人参成分のサイトカイン(IL-1β)分泌抑制作用","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"田七人参成分のサイトカイン(IL-1β)分泌抑制作用"},{"subitem_title":"Inhibitory effect of Panax notoginseng methanol extract (denchi) on the cytokine IL-1β release from PBMC cells","subitem_title_language":"en"}]},"item_type_id":"4","owner":"3","path":["1135"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-04"},"publish_date":"2017-10-04","publish_status":"0","recid":"15353","relation_version_is_last":true,"title":["田七人参成分のサイトカイン(IL-1β)分泌抑制作用"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-07-28T00:33:50.005969+00:00"}