{"created":"2023-07-27T06:30:22.534723+00:00","id":15372,"links":{},"metadata":{"_buckets":{"deposit":"ef3e17cc-773e-42a5-944b-97a8b6373220"},"_deposit":{"created_by":3,"id":"15372","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"15372"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00015372","sets":["4161:3020:3021"]},"author_link":["27925","27924","27926","76362","196","2309","26716","63"],"item_4_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-04-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"7","bibliographicPageEnd":"1052","bibliographicPageStart":"1045","bibliographicVolumeNumber":"79","bibliographic_titles":[{"bibliographic_title":"Biochemical pharmacology"}]}]},"item_4_creator_33":{"attribute_name":"著者別表示","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"中嶋, 美紀 "}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"深見, 達基"}],"nameIdentifiers":[{},{}]}]},"item_4_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Human CYP2E1 is one of the pharmacologically and toxicologically important cytochrome P450 isoforms. Earlier studies have reported that the CYP2E1 expression is extensively regulated by post-transcriptional and post-translational mechanisms, but the molecular basis remains unclear. In the present study, we examined the possibility that microRNA may be involved in the post-transcriptional regulation of human CYP2E1. In silico analysis identified a potential recognition element of miR-378 (MRE378) in the 3'-untranslated region (UTR) of human CYP2E1 mRNA. Luciferase assays using HEK293 cells revealed that the reporter activity of the plasmid containing the MRE378 was decreased by co-transfection of precursor miR-378, indicating that miR-378 functionally recognized the MRE378. We established two HEK293 cell lines stably expressing human CYP2E1 including or excluding 3'-UTR. When the precursor miR-378 was transfected into the cells expressing human CYP2E1 including 3'-UTR, the CYP2E1 protein level and chlorzoxazone 6-hydroxylase activity were significantly decreased, but were not in the cells expressing CYP2E1 excluding 3'-UTR. In both cell lines, the CYP2E1 mRNA levels were decreased by overexpression of miR-378, but miR-378 did not affect the stability of CYP2E1 mRNA. In a panel of 25 human livers, no positive correlation was observed between the CYP2E1 protein and CYP2E1 mRNA levels, supporting the post-transcriptional regulation. Interestingly, the miR-378 levels were inversely correlated with the CYP2E1 protein levels and the translational efficiency of CYP2E1. In conclusion, we found that human CYP2E1 expression is regulated by miR-378, mainly via translational repression. This study could provide new insight into the unsolved mechanism of the post-transcriptional regulation of CYP2E1. Copyright 2009 Elsevier Inc. All rights reserved.","subitem_description_type":"Abstract"}]},"item_4_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学ナノ生命科学研究所 / 金沢大学医薬保健研究域薬学系","subitem_description_type":"Other"}]},"item_4_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24517/00015359","subitem_identifier_reg_type":"JaLC"}]},"item_4_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_4_relation_12":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.bcp.2009.11.015","subitem_relation_type_select":"DOI"}}]},"item_4_relation_28":{"attribute_name":"関連URI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"http://www.elsevier.com/locate/issn/00062952","subitem_relation_type_select":"URI"}}]},"item_4_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AA00564486","subitem_source_identifier_type":"NCID"}]},"item_4_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0006-2952","subitem_source_identifier_type":"ISSN"}]},"item_4_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Mohri, Takuya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakajima, Miki"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Fukami, Tatsuki"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Takamiya, Masataka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Aoki, Yasuhiro"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yokoi, Tsuyoshi"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-04"}],"displaytype":"detail","filename":"PH-PR-NAKAJIMA-M-1045.pdf","filesize":[{"value":"315.8 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"PH-PR-NAKAJIMA-M-1045.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/15372/files/PH-PR-NAKAJIMA-M-1045.pdf"},"version_id":"c012e29b-2cd9-43dd-a477-24050c30cbd6"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Human CYP2E1 is regulated by miR-378.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Human CYP2E1 is regulated by miR-378."}]},"item_type_id":"4","owner":"3","path":["3021"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-04"},"publish_date":"2017-10-04","publish_status":"0","recid":"15372","relation_version_is_last":true,"title":["Human CYP2E1 is regulated by miR-378."],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-07-27T10:11:54.776896+00:00"}