@article{oai:kanazawa-u.repo.nii.ac.jp:00015389,
 author = {Kameda, Takayuki and Akiyama, Ayuko and Toriba, Akira and Tachikawa, Chihiro and Yoshita, Morio and Tang, Ning and Hayakawa, Kazuichi},
 issue = {1},
 journal = {Journal of Health Science = 衛生化学},
 month = {Feb},
 note = {Endocrine disrupting activities of three isomers of monohydroxylated 1-nitropyrene (1-NP) [3-, 6-, and 8-hydroxy-1-nitropyrenes (OHNPs)] were evaluated for the first time by yeast two-hybrid assay. OHNPs, which are not only metabolites of 1-NP but are also found in airborne particles, did not exhibit androgenic activity but exhibited estrogenic, antiestrogenic, and antiandrogenic activities. 6-OHNP showed the strongest estrogenic activity among the three OHNP isomers examined in this study. Concentrations of the OHNP isomers that gave 10% of activity of 1.0 × 10-6 M 17β-estradiol (E2) were as follows: 3-OHNP, 6.0 × 10 -7 M; 6-OHNP, 6.0 × 10-8 M; 8-OHNP, 9.0 × 10-7 M. On the contrary, 8-OHNP exhibited the strongest antiestrogenic and antiandrogenic activities of the three isomers. 1.0 × 10-6 M of 8-OHNP inhibited 32 and 90% of β-galactosidase activity induced by 1.0 × 10-9 M of E2 and 1.0 × 10-8 M of 5α-dihydrotestosterone (DHT), respectively. These findings point out the necessity for detailed investigation of environmental sources and distributions of OHNPs as well as the parent 1-NP.},
 pages = {118--122},
 title = {Evaluation of Endocrine Disrupting Activities of Monohydroxylated Derivatives of 1-nitropyrene by Yeast Two-hybrid Assay},
 volume = {54},
 year = {2008}
}