@article{oai:kanazawa-u.repo.nii.ac.jp:00016747,
 author = {保野, 由紀子},
 issue = {2},
 journal = {金沢大学十全医学会雑誌 = Journal of the Juzen Medical Society},
 month = {Jun},
 note = {Background: Epithelial cells of endometriotic tissues are difficult to propagate in vitro as experimental material is scarce owing to their limited life span. However, there is an increasing concern regarding their malignant transformation in ovaries. The present study sought to generate their stable culture system.Methods and Results :Purified epithelial cells isolated from ovarian endometriomas using microscopic manipulation were successfully immortalised by combinatorial transfection of human cyclinD1, cdk4 and human telomerase reverse transcriptase (hTERT) genes, whereas the introduction of hTERT alone, or together with cdk4, was insufficient for immortalisation, leading to cellular senescence. We confirmed stable cytokeratin expression in the immortalised cells, proving their epithelial origin. These cells expressed progesterone receptor B and showed significant growth inhibition by various progestins. Oestrogen receptor (ER) expression was detected in these cells, albeit at low levels. Additional overexpression of ERα generated stable cells with oestrogen-dependent growth activation. Soft-agar colony formation assay and nude mice xenograft experiments demonstrated that these cells, even those with additional inactivation of p53, did not have transformed phenotypes. Conclusion: We for the first time generated immortalised epithelial cells from ovarian endometrioma that retained sex steroid responsiveness. These cells are invaluable tools not only for the consistent in vitro work but also for the study of molecular pathogenesis or carcinogenesis of endometriosis. © 2012 Cancer Research UK All rights reserved., [総説 / Reviews][博士課程優秀論文] 第11回 高安賞優秀論文賞受賞論文, British Journal of Cancer 106(6), pp.1205-1213(2012年2月掲載), Bono, Y., Kyo, S., Takakura, M., Maida, Y., Mizumoto, Y., Nakamura, M., Nomura, K., Kiyono, T., Inoue, M. "Creation of immortalised epithelial cells from ovarian endometrioma.", http://dx.doi.org/10.1038/bjc.2012.26},
 pages = {37--38},
 title = {子宮内膜症性卵巣嚢胞から分離した子宮内膜症上皮不死化細胞株の樹立},
 volume = {122},
 year = {2013}
}