{"created":"2023-07-27T06:31:55.306164+00:00","id":17528,"links":{},"metadata":{"_buckets":{"deposit":"3df3e431-ff12-48a5-8700-6bae89c0ce2e"},"_deposit":{"created_by":3,"id":"17528","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"17528"},"status":"published"},"_oai":{"id":"oai:kanazawa-u.repo.nii.ac.jp:00017528","sets":["1132:1137:1270:1357"]},"author_link":["31126"],"item_7_biblio_info_8":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1996-02-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"70","bibliographicPageStart":"54","bibliographicVolumeNumber":"105","bibliographic_titles":[{"bibliographic_title":"金沢大学十全医学会雑誌"}]}]},"item_7_description_16":{"attribute_name":"その他の識別子","attribute_value_mlt":[{"subitem_description":"1996244250","subitem_description_type":"Other"}]},"item_7_description_21":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Slc/ICRマウスを用いて,胆嚢,総胆管,肝門部胆管,末梢部胆管の解剖学的区分からの胆道系上皮細胞の長期培養を行うことに成功した. 1)胆道系上皮細胞の初代培養をコラーゲンゲル培地上で行い,間葉系細胞の混入のない上皮成分からなる部分を切り取り,継代することにより胆道系上皮細胞の純粋培養に成功した.3週間毎に継代し,胆嚢,総胆管,肝門部胆管は10代目まで,末梢部胆管は6代目まで観察し得た. 2)培養細胞はコラーゲンゲル上を単層性に敷石状に増殖進展し,コロニーの中心部では立方状～円柱状の形態を示し,辺縁部では扁平な形態を示した.粘液の産生は殆どみられなかった.各胆道系培養細胞はγ-GTP染色がび漫性に陽性であった.電顕による観察では管腔側には微絨毛が密生し,細胞間には接着装置や嵌合ヒダがみられ,生体内の胆道系上皮としての特徴を備えていた","subitem_description_type":"Abstract"}]},"item_7_description_5":{"attribute_name":"提供者所属","attribute_value_mlt":[{"subitem_description":"金沢大学 医 第2病理","subitem_description_type":"Other"}]},"item_7_publisher_17":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"金沢大学十全医学会"}]},"item_7_source_id_11":{"attribute_name":"NCID","attribute_value_mlt":[{"subitem_source_identifier":"AN00044397","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0022-7226","subitem_source_identifier_type":"ISSN"}]},"item_7_version_type_25":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"片柳, 和義"}],"nameIdentifiers":[{"nameIdentifier":"31126","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-10-04"}],"displaytype":"detail","filename":"AN00044397-105-006.pdf","filesize":[{"value":"4.7 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AN00044397-105-006.pdf","url":"https://kanazawa-u.repo.nii.ac.jp/record/17528/files/AN00044397-105-006.pdf"},"version_id":"3b5901a1-2039-472d-99bc-aaa6ca3ff0d5"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"上皮細胞(培地・培養)","subitem_subject_scheme":"Other"},{"subitem_subject":"胆道","subitem_subject_scheme":"Other"},{"subitem_subject":"マウス","subitem_subject_scheme":"Other"},{"subitem_subject":"マウス","subitem_subject_scheme":"Other"},{"subitem_subject":"動物","subitem_subject_scheme":"Other"},{"subitem_subject":"in vitro","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"マウス胆道系上皮細胞の区分別培養法の確立","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"マウス胆道系上皮細胞の区分別培養法の確立"}]},"item_type_id":"7","owner":"3","path":["1357"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-10-04"},"publish_date":"2017-10-04","publish_status":"0","recid":"17528","relation_version_is_last":true,"title":["マウス胆道系上皮細胞の区分別培養法の確立"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-07-28T00:02:02.444839+00:00"}