@article{oai:kanazawa-u.repo.nii.ac.jp:00026627,
 author = {Nomura, Masaaki and Ichimatsu, Daisuke and Moritani, Shuzo and Koyama, Ichiko and Dong, Zigang and Yokogawa, Koichi and Miyamoto, Kenichi},
 issue = {1},
 journal = {Molecular Carcinogenesis},
 month = {Sep},
 note = {We found that caffeine significantly inhibited epidermal growth factor (EGF)- and 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced cell transformation in the JB6 mouse epidermal cell line. The tumor promoter-induced cell transformation was also blocked by treatment with an adenosine A1 receptor antagonist, 8-phenyltheophylline (8-PTH). Caffeine slightly attenuated activation of EGF-induced activator protein 1 (AP-1) activation, which play important roles in cell transformation, but only at the highest concentration examined (1 mM). Interestingly, pretreatment with caffeine suppressed EGF-induced phosphorylation and activation of Akt and ribosomal p70 S6 protein kinase (p70 S6K), a target of Akt, without inhibiting phosphatidylinositol 3-kinase (PI3K) activation. The inhibition of Akt activation of caffeine was not a result of its adenosine receptor antagonism. Because Akt plays a key role in signal transduction pathways leading to cell proliferation and apoptosis, our results provide novel insight into possible mechanisms of the chemotherapeutic effect of caffeine. © 2005 Wiley-Liss, Inc., 金沢大学医学部附属病院薬剤部},
 pages = {67--76},
 title = {Inhibition of epidermal growth factor-induced cell transformation and Akt activation by caffeine},
 volume = {44},
 year = {2005}
}